Difference between revisions of "Part:BBa K1613014"

 
 
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__NOTOC__
 
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<partinfo>BBa_K1613014 short</partinfo>
 
<partinfo>BBa_K1613014 short</partinfo>
  
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to detect cadmium
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This part is designed to proceed cadmium detection in E.coli. ZinTp(YodA) K896008 is a promoter which is designed by NYMU in 2013. ZinTp (pYodA) is a promoter which expresses the downstream gene in the presence of cadmium ion. The activity of this promoter is specific affect by cadmium ion and won’t be induced by other ions like zinc, copper, cobalt, and nickel. Bacterial accommodation to moderate concentrations of cadmium is accompanied by transient activation of general stress proteins as well as a sustained induction of other proteins of hitherto unknown functions. One of the latter proteins was previously identified as the product of the Escherichia coli yodA ORF. The yodA ORF encodes 216 aa residues (the YodA protein) and the increased synthesis of YodA during cadmium stress was found probably to be a result of transcriptional activation from one single promoter upstream of the structural yodA gene. Analysis of a transcriptional gene fusion, ZinTp, yodA demonstrated that basal expression of yodA is low during exponential growth and expression is increased greater than 50-fold by addition of cadmium to growing cells. However, challenging cells with additional metals such as zinc, copper, cobalt and nickel did not increase the level of yodA expression. A defined start point initiates 8 bp downstream of the k10 region with the sequence CAT frequently occurred in the RNA start points of natural promoters. The k10 and k35 elements of ZinTp show good similarity to the consensus sequences for these elements. However, the ZinTp includes a long spacer region (19 bp) between these two elements, compared with the normal spacing of 17p1 bp in a highly expressed σ@3 promoter. Such extended spacer regions are common in metal-responsive promoters, for instance 19 bp for Pmer of Tn501 and 20 bp for PzntA of E. coli. Both these promoters are regulated by MerR-like transcriptional activators which bind to an inverted repeat sequence located between the k10 and k35 promoter regions. HSNU-TAIPEI used this part to detect cadmium ion in recycled oil.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 20:36, 26 September 2015

It is a new part which can detect cadmium ions in liquid.

This part is designed to proceed cadmium detection in E.coli. ZinTp(YodA) K896008 is a promoter which is designed by NYMU in 2013. ZinTp (pYodA) is a promoter which expresses the downstream gene in the presence of cadmium ion. The activity of this promoter is specific affect by cadmium ion and won’t be induced by other ions like zinc, copper, cobalt, and nickel. Bacterial accommodation to moderate concentrations of cadmium is accompanied by transient activation of general stress proteins as well as a sustained induction of other proteins of hitherto unknown functions. One of the latter proteins was previously identified as the product of the Escherichia coli yodA ORF. The yodA ORF encodes 216 aa residues (the YodA protein) and the increased synthesis of YodA during cadmium stress was found probably to be a result of transcriptional activation from one single promoter upstream of the structural yodA gene. Analysis of a transcriptional gene fusion, ZinTp, yodA demonstrated that basal expression of yodA is low during exponential growth and expression is increased greater than 50-fold by addition of cadmium to growing cells. However, challenging cells with additional metals such as zinc, copper, cobalt and nickel did not increase the level of yodA expression. A defined start point initiates 8 bp downstream of the k10 region with the sequence CAT frequently occurred in the RNA start points of natural promoters. The k10 and k35 elements of ZinTp show good similarity to the consensus sequences for these elements. However, the ZinTp includes a long spacer region (19 bp) between these two elements, compared with the normal spacing of 17p1 bp in a highly expressed σ@3 promoter. Such extended spacer regions are common in metal-responsive promoters, for instance 19 bp for Pmer of Tn501 and 20 bp for PzntA of E. coli. Both these promoters are regulated by MerR-like transcriptional activators which bind to an inverted repeat sequence located between the k10 and k35 promoter regions. HSNU-TAIPEI used this part to detect cadmium ion in recycled oil.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 663
    Illegal AgeI site found at 775
  • 1000
    COMPATIBLE WITH RFC[1000]