Difference between revisions of "Part:BBa K1602002"
 
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         <h1>Aconitate hydratase - acnA</h1>
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         <h1>B0034-yjhG</h1>
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         <table>
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         This composite represents the <small>D</small>-xylonic acid dehydratases <i>yjhG</i> plus the ribosomal binding site B0034.
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            <colgroup width=50% span="2">
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            <tr>
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                <td>
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                <b>acnA</b> is a gene, coding for a citrate-isomerase that occurs in <i>E.coli</i>(e.g.). It is a main part of the TCA-cycle and is responsible for the isomerization of citrate to cis-aconitate via isocitrate.
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<!--<br>
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                FUELLTEXT
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                <br>
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                <br>
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-->
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                </td>
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                <td>
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<div style="width: 300px; height: 300px; align: center; margin-left: 100px; margin-right:100px">
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<video controls loop autoplay>
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  <source src="https://static.igem.org/mediawiki/2015/e/e4/GltA_low_animated.mp4" type="video/mp4">
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</video>
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</div>
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                </td>
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            </tr>
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                    <!-- BLANK -->
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                </td>
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                <td>
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                    <b>Figure 1</b> aconitate-isomerase (coded by acnA)
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                </td>
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            </tr>
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            </colgroup>
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        </table>
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===<h2>Usage</h2>===
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===<h2>Characteristics</h2>===
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        This part is a composite of one coding gene and a strong RBS (<a href="/Part:BBa_B0034">BBa_B0034</a>).
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        <br>
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        <br>
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        This part makes it possible to establish an ethyleneglycol producing pathway in other hosts than <i>E. coli</i> if its combined with other enzymes of the pathway <a href="https://parts.igem.org/Part:BBa_K1602017">(BBa_K1602017)</a> <a href="https://parts.igem.org/Part:BBa_K1602014">(BBa_K1602014)</a> <a href="https://parts.igem.org/Part:BBa_K1602015">(BBa_K1602015)</a>. (1)
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        <br>
 
         <br>
 
         <br>
 
         <table>
 
         <table>
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             <colgroup width=50% span="4">
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             <colgroup width=33% span=3>
 
             <tr>
 
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                 <td>
 
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                     Molecular Weight
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                     <ul>
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                        <li>yjhG</li>
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                        <li>RBS-B0034</li>
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                    </ul>
 
                 </td>
 
                 </td>
 
                 <td>
 
                 <td>
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                     97677.22
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                     <ul>
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                        <li><a href="https://parts.igem.org/Part:BBa_K1602012">BBa_K1602012</a></li>
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                        <li><a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a></li>
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                    </ul>
 
                 </td>
 
                 </td>
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            </tr>
 
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            <tr>
 
 
                 <td>
 
                 <td>
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                     Residues
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                     <div align="center" valign="middle"><img class="transparent shrinkToFit" alt="https://static.igem.org/mediawiki/parts/e/e5/TU_Darmstadt_plasmid-card_B0034-yjhG.png" src="https://static.igem.org/mediawiki/parts/e/e5/TU_Darmstadt_plasmid-card_B0034-yjhG.png" height="227" width="255"></div>
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                </td>
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                <td>
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                    891
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            </tr>
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            <tr>
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                <td>
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                    Charge
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                </td>
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                <td>
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                    -9.0
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                </td>
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            </tr>
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            <tr>
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                <td>
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                    Isoelectric Point
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                </td>
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                <td>
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                    109.627
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                </td>
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            </tr>
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            <tr>
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                <td>
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                    A280 Molar Extinction Coefficients
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                </td>
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                <td>
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                    114710 (reduced)  115085 (cystine bridges)
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                </td>
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            </tr>
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            <tr>
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                <td>
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                    [Data taken from PEPSTATS]
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                 </td>
 
                 </td>
 
             </tr>
 
             </tr>
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             </colgroup>
 
             </colgroup>
 
         </table>
 
         </table>
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===<h2>Sequence and Features</h2>===
 
===<h2>Sequence and Features</h2>===
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<partinfo>BBa_K1602001 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1602002 SequenceAndFeatures</partinfo>
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<br>
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===References===
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1. Liu H, Ramos KR, Valdehuesa KN, Nisola GM, Lee WK, Chung WJ. Biosynthesis of ethylene glycol in Escherichia coli. Appl Microbiol Biotechnol. 2013;97(8):3409-17.

Latest revision as of 22:34, 18 September 2015

B0034-yjhG

This composite represents the D-xylonic acid dehydratases yjhG plus the ribosomal binding site B0034.

Usage

This part is a composite of one coding gene and a strong RBS (BBa_B0034).

This part makes it possible to establish an ethyleneglycol producing pathway in other hosts than E. coli if its combined with other enzymes of the pathway (BBa_K1602017) (BBa_K1602014) (BBa_K1602015). (1)

  • yjhG
  • RBS-B0034
https://static.igem.org/mediawiki/parts/e/e5/TU_Darmstadt_plasmid-card_B0034-yjhG.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1016
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1921
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 179


References

1. Liu H, Ramos KR, Valdehuesa KN, Nisola GM, Lee WK, Chung WJ. Biosynthesis of ethylene glycol in Escherichia coli. Appl Microbiol Biotechnol. 2013;97(8):3409-17.