Difference between revisions of "Part:BBa K1800001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This part was constructed with the alpha secretion signal coding sequence taken from a pGAPza vector. RFC 25 prefix and suffix were added to enable the creation of fusion proteins that can be secreted. | + | This part was constructed with the alpha secretion signal coding sequence taken from a pGAPza vector. RFC 25 prefix and suffix were added to enable the creation of fusion proteins that can be secreted outside the cell. |
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===Source=== | ===Source=== | ||
Latest revision as of 21:29, 1 September 2015
Alpha-Factor Secretion Signal
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 244
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was constructed with the alpha secretion signal coding sequence taken from a pGAPza vector. RFC 25 prefix and suffix were added to enable the creation of fusion proteins that can be secreted outside the cell.
Source
The coding sequence for this part was taken from the pGAPza pichia pastoris expression vector.