Difference between revisions of "Part:BBa K1758330:Design"
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<partinfo>BBa_K1758330 short</partinfo> | <partinfo>BBa_K1758330 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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+ | We used the codon optimized, synthesized Sequence of pbrR from iGEM Team Brown 2007 (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I721003" target="_blank"> BBa_I721003</a>) under the control of a constitutive Primer(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I721003" target="_blank"> BBa_I721003</a>). We designed the used primes, in specially split primers, for Gibson Assembly to ligate the constitutive Promoter and pbrR with pSB1C3. For this aim we designed four primers for the generation of homologous overlaps between the synthesized constitutive promoter+ pbrR and the pSB1C3: | ||
+ | <p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_rev" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_rev" target="_blank">cm_rev</a></p><p>TATACGCAAGGCGACAAG</p> | ||
+ | <p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers# pSB1C3_kPrm_fwd" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers# pSB1C3_kPrm_fwd" target="_blank"> pSB1C3_kPrm_fwd</a></p><p>CTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA </p> | ||
+ | <p> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pSB1C3_rcnR_rev" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pSB1C3_rcnR_rev" target="_blank"> pSB1C3_rcnR_rev </a></p><p>GAAAGTCCTTGATTCGTACATCAAATGACTCTAGAAGCGGCCGCGAAT </p> | ||
+ | <p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_fwd" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_fwd" target="_blank">cm_fwd</a><p>CGGCATCAGCACCTTGTC</p> | ||
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+ | </html> | ||
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===Source=== | ===Source=== | ||
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+ | Synthesized, codon optimized gene, designed after (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I721003" target="_blank"> BBa_I721003</a>) <i>ralstonia metallidurans </i> synthesized by IDT | ||
+ | </html> | ||
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===References=== | ===References=== |
Latest revision as of 23:33, 18 September 2015
Lead repressor under control of constitutive promoter and strong RBS
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 465
Illegal NheI site found at 488 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used the codon optimized, synthesized Sequence of pbrR from iGEM Team Brown 2007 ( BBa_I721003) under the control of a constitutive Primer( BBa_I721003). We designed the used primes, in specially split primers, for Gibson Assembly to ligate the constitutive Promoter and pbrR with pSB1C3. For this aim we designed four primers for the generation of homologous overlaps between the synthesized constitutive promoter+ pbrR and the pSB1C3:
TATACGCAAGGCGACAAG
CTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA
GAAAGTCCTTGATTCGTACATCAAATGACTCTAGAAGCGGCCGCGAAT
CGGCATCAGCACCTTGTC
Source
Synthesized, codon optimized gene, designed after ( BBa_I721003) ralstonia metallidurans synthesized by IDT