Difference between revisions of "Part:BBa K1621003:Design"

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===References===
 
===References===
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Rummel A, Bade S, Alves J, Bigalke H, Binz J, Two carbohydrate binding sites in the HCC-domain of tetanus neurotoxin are required for toxicity (2003). ''J. Mol. Biol.'' 326 (3), 835–847.
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Yu R, Hou L, Yu C, Liu S, Ren J, Fang T, Zhang X, Chen W, Enhanced expression of soluble recombinant tetanus neurotoxin Hc in ''Escherichia coli'' as a tetanus vaccine candidate (2011). ''Immunobiology'' 216, 485–490.

Latest revision as of 06:30, 30 August 2015


TeNT_Hc - C-terminal part of the heavy chain (tetanus toxin)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 430
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for expression in E.coli with the codon optimization tool from IDT.


Source

The sequence for this part was obtained from Yu et al. (2010) and synthesized by Integrated DNA Technolgies.

References

Rummel A, Bade S, Alves J, Bigalke H, Binz J, Two carbohydrate binding sites in the HCC-domain of tetanus neurotoxin are required for toxicity (2003). J. Mol. Biol. 326 (3), 835–847.

Yu R, Hou L, Yu C, Liu S, Ren J, Fang T, Zhang X, Chen W, Enhanced expression of soluble recombinant tetanus neurotoxin Hc in Escherichia coli as a tetanus vaccine candidate (2011). Immunobiology 216, 485–490.