Difference between revisions of "Part:BBa K1602000"
 
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         <h1>Inducible itaconic acid producing operon</h1>
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         <h1>B0034-xylB</h1>
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         <b>Itaconic acid</b> is an organic, dicarboxylic acid that is biotechnologically synthesized most commonly in Aspergillus terreus. It is derived          from citric acid via 2 intermediates and a final decarboxylation.
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         This composite represents the <small>D</small>-xylose dehydrogenase <i>xylB</i> plus the ribosomal binding site B0034.
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===<h2>Usage</h2>===
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        This part is a composite of one coding gene and a strong RBS (<a href="/Part:BBa_B0034">BBa_B0034</a>).
 
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        To enable this pathway in <i>Escherichia coli</i> it is necessary to introduce 3 genes. 2 of which are already established in the citric acid cycle            and one that is from <i>Apergillus terreus</i>, a cis-aconitate decarboxylase (cadA).
 
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        <img style="width: 900px; height: 110px; margin-left: 15px; margin-right: 15px;" alt="" src="https://static.igem.org/mediawiki/2015/a/ae/Itaconic_acid_chemistry.jpg">
 
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            <b>Figure 1</b> Reaction scheme of the itaconic acid producing operon. The substrates for the reaction are acetyl-coa and oxaloacetate. To                    simplify the process we are only looking at oxaloacetate right now. Oxaloacetate is metabolized to itaconic acid in 3 steps.
 
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        <h2>Usage</h2>
 
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            This part is a composite of three genes, each with a strong RBS (<a href="/Part:BBa_B0034">BBa_B0034</a>) and all of them under control of one            T7 Promoter (<a href="/Part:BBa_K1497017">BBa_K1497017</a>).
 
 
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        We used this part for our <small>D</small>-xylonic acid producing operon <a href="https://parts.igem.org/Part:BBa_K1602017">(BBa_K1602017)</a>
 
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                         <li class="block-10vi">citrate synthase - gltA                     <a href="/Part:BBa_K1602001">(BBa_K1602001)</a></li>
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                         <li>xylB</li>
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                         <li class="block-10vi">aconitate hydratase 1 - acnA             <a href="/Part:BBa_K1602002">(BBa_K1602002)</a></li>
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                         <li>RBS-B0034</li>
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                        <li class="block-10vi">cis-aconitate decarboxylase - cadA            <a href="/Part:BBa_K1602003">(BBa_K1602003)</a></li>  
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                     <div style="float: right;"><img style="width: 400px; height: 120px;" alt="" src="https://static.igem.org/mediawiki/2015/a/a1/Itaconic_acid_gene_operon.png"> </div>      
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                </td>
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                        <li><a href="https://parts.igem.org/Part:BBa_K1602009">BBa_K1602009</a></li>
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                        <li><a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a></li>
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                     <b>Figure 2</b> Genetic map of the itaconic acid producing operon with T7 promoter. This brick directs the flux towards and finally                            enables <i>E.Coli</i> BL21 cells to synthesize itaconic acid in presence of the inductor IPTG.
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                     <div align="center" valign="middle"><img class="transparent shrinkToFit" alt="https://static.igem.org/mediawiki/parts/5/58/TU_Darmstadt_plasmid_cardB0034-xylB.png" src="https://static.igem.org/mediawiki/parts/5/58/TU_Darmstadt_plasmid_cardB0034-xylB.png" height="227" width="255"></div>
 
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<h4>Sequence and Features</h4>
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===<h2>Sequence and Features</h2>===
 
<partinfo>BBa_K1602000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1602000 SequenceAndFeatures</partinfo>

Latest revision as of 21:45, 18 September 2015

B0034-xylB

This composite represents the D-xylose dehydrogenase xylB plus the ribosomal binding site B0034.

Usage

This part is a composite of one coding gene and a strong RBS (BBa_B0034).

We used this part for our D-xylonic acid producing operon (BBa_K1602017)

  • xylB
  • RBS-B0034
https://static.igem.org/mediawiki/parts/5/58/TU_Darmstadt_plasmid_cardB0034-xylB.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]