Difference between revisions of "Part:BBa K1602000"
 
(16 intermediate revisions by 3 users not shown)
Line 9: Line 9:
 
     </head>
 
     </head>
 
     <body>
 
     <body>
−
         <h1>Inducible itaconic acid producing operon</h1>
+
         <h1>B0034-xylB</h1>
−
         <b>Itaconic acid</b> is an organic, dicarboxylic acid that is biotechnologically synthesized most commonly in Aspergillus terreus. It is derived          from citric acid via 2 intermediates and a final decarboxylation.
+
         This composite represents the <small>D</small>-xylose dehydrogenase <i>xylB</i> plus the ribosomal binding site B0034.
 +
    </body>
 +
</html>
 +
===<h2>Usage</h2>===
 +
<html>
 +
    <body>
 +
        This part is a composite of one coding gene and a strong RBS (<a href="/Part:BBa_B0034">BBa_B0034</a>).
 
         <br>
 
         <br>
−
        To enable this pathway in <i>Escherichia coli</i> it is necessary to introduce 3 genes. 2 of which are already established in the citric acid cycle            and one that is from <i>Apergillus terreus</i>, a cis-aconitate decarboxylase (cadA).
 
−
        <br>
 
−
        <br>
 
−
        <img style="width: 900px; height: 110px; margin-left: 15px; margin-right: 15px;" alt="" src="pictures/chem_way.jpg">
 
−
        <p style="width: 900px; margin-left: 15px; margin-right: 15px;" alt="">
 
−
            <b>Figure 1</b> Reaction scheme of the itaconic acid producing operon. The substrates for the reaction are acetyl-coa and oxaloacetate. To                    simplify the process we are only looking at oxaloacetate right now. Oxaloacetate is metabolized to itaconic acid in 3 steps.
 
−
        </p>
 
−
        <br>
 
−
        <br>
 
−
        <h2>Usage</h2>
 
−
            This part is a composite of three genes, each with a strong RBS (<a href="/Part:BBa_B0034">BBa_B0034</a>) and all of them under control of one            T7 Promoter (<a href="/Part:BBa_K1497017">BBa_K1497017</a>).
 
 
         <br>
 
         <br>
 +
        We used this part for our <small>D</small>-xylonic acid producing operon <a href="https://parts.igem.org/Part:BBa_K1602017">(BBa_K1602017)</a>
 
         <br>
 
         <br>
 
         <br>
 
         <br>
 
         <table>
 
         <table>
−
             <colgroup width=50% span="2">
+
             <colgroup width=33% span=3>
 
             <tr>
 
             <tr>
 
                 <td>
 
                 <td>
 
                     <ul>
 
                     <ul>
−
                         <li class="block-10vi">citrate synthase - gltA                     <a href="/Part:BBa_K1033001">(BBa_K1033001)</a></li>
+
                         <li>xylB</li>
−
                         <li class="block-10vi">aconitate hydratase 1 - acnA             <a href="/Part:BBa_K1033000">(BBa_K1033000)</a></li>
+
                         <li>RBS-B0034</li>
−
                        <li class="block-10vi">cis-aconitate decarboxylase - cadA            <a href="/Part:BBa_K1497000">(BBa_K1497100)</a></li>  
+
 
                     </ul>
 
                     </ul>
 
                 </td>
 
                 </td>
 
                 <td>
 
                 <td>
−
                     <div style="float: right;"><img style="width: 400px; height: 120px;" alt="" src="pictures/gene_operon.png"> </div>      
+
                     <ul>
−
                </td>
+
                        <li><a href="https://parts.igem.org/Part:BBa_K1602009">BBa_K1602009</a></li>
−
            </tr>
+
                        <li><a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a></li>
−
            <tr>
+
                     </ul>
−
                <td>
+
−
                     <!-- BLANK -->
+
 
                 </td>
 
                 </td>
 
                 <td>
 
                 <td>
−
                     <b>Figure 2</b> Genetic map of the itaconic acid producing operon with T7 promoter. This brick directs the flux towards and finally                            enables <i>E.Coli</i> BL21 cells to synthesize itaconic acid in presence of the inductor IPTG.
+
                     <div align="center" valign="middle"><img class="transparent shrinkToFit" alt="https://static.igem.org/mediawiki/parts/5/58/TU_Darmstadt_plasmid_cardB0034-xylB.png" src="https://static.igem.org/mediawiki/parts/5/58/TU_Darmstadt_plasmid_cardB0034-xylB.png" height="227" width="255"></div>
 
                 </td>
 
                 </td>
 
             </tr>
 
             </tr>
 +
           
 
             </colgroup>
 
             </colgroup>
 
         </table>
 
         </table>
−
       
+
    <br>
 +
    <br>
 +
   
 
     </body>
 
     </body>
 
</html>  
 
</html>  
  
−
<h4>Sequence and Features</h4>
+
===<h2>Sequence and Features</h2>===
−
<partinfo>BBa_K1497016 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K1602000 SequenceAndFeatures</partinfo>

Latest revision as of 21:45, 18 September 2015

B0034-xylB

This composite represents the D-xylose dehydrogenase xylB plus the ribosomal binding site B0034.

Usage

This part is a composite of one coding gene and a strong RBS (BBa_B0034).

We used this part for our D-xylonic acid producing operon (BBa_K1602017)

  • xylB
  • RBS-B0034
https://static.igem.org/mediawiki/parts/5/58/TU_Darmstadt_plasmid_cardB0034-xylB.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]