Difference between revisions of "Part:BBa K1602000"

 
 
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<partinfo>BBa_K1602000 short</partinfo>
 
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<partinfo>BBa_IA</partinfo>
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        <h1>B0034-xylB</h1>
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<b>Itaconic acid</b> is an organic, dicarboxylic acid that is biotechnologically synthesized in Aspergillus terreus. It is derived from citric acid        via 2 intermediates and a final decarboxylation.
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        This composite represents the <small>D</small>-xylose dehydrogenase <i>xylB</i> plus the ribosomal binding site B0034.
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===<h2>Usage</h2>===
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To enable this pathway in Escherichia coli it is necessary to introduce 3 genes. 2 of which are already established in the citric acid cycle and           one that is from Apergillus terreus, a cis-aconitate decarboxylase (cadA).
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        This part is a composite of one coding gene and a strong RBS (<a href="/Part:BBa_B0034">BBa_B0034</a>).
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        We used this part for our <small>D</small>-xylonic acid producing operon <a href="https://parts.igem.org/Part:BBa_K1602017">(BBa_K1602017)</a>
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<b>Figure 1</b> Reaction scheme of the itaconic acid producing operon. The substrates for the reaction are acetyl-coa and oxaloacetate. To                simplify the process we are only looking at oxaloacetate right now. Oxaloacetate is metabolized to itaconic acid in 3 steps.
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                        <li>xylB</li>
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                        <li>RBS-B0034</li>
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===Usage===
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                        <li><a href="https://parts.igem.org/Part:BBa_K1602009">BBa_K1602009</a></li>
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                        <li><a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a></li>
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                    <div align="center" valign="middle"><img class="transparent shrinkToFit" alt="https://static.igem.org/mediawiki/parts/5/58/TU_Darmstadt_plasmid_cardB0034-xylB.png" src="https://static.igem.org/mediawiki/parts/5/58/TU_Darmstadt_plasmid_cardB0034-xylB.png" height="227" width="255"></div>
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      <td style="padding: 0cm 5.4pt; width: 336.7pt; height: 214.9pt;"> This part is a composite of three genes, each with a strong RBS (<a                      href="/Part:BBa_B0034">BBa_B0034</a>) and all of them under control of one T7 Promoter (<a href="/Part:BBa_K1497017">BBa_K1497017</a>).
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===<h2>Sequence and Features</h2>===
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              <li class="block-10vi">citrate synthase - gltA                     <a href="/Part:BBa_K1033001">(BBa_K1033001)</a></li>
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              <li class="block-10vi">aconitate hydratase 1 - acnA             <a href="/Part:BBa_K1033000">(BBa_K1033000)</a></li>
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              <li class="block-10vi">cis-aconitate decarboxylase - cadA            <a href="/Part:BBa_K1497000">(BBa_K1497100)</a></li>
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          <font color="#FFFFFF">iGEM TU Darmstadt 2014 :)</font>
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              <b>Figure 2</b>
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              Genetic map of the itaconic acid producing operon with T7 promoter. This brick directs the flux towards and finally enables <i>E.Coli</i>                   BL21 cells to synthesize itaconic acid in presence of the inductor IPTG.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1602000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1602000 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
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<partinfo>BBa_K1602000 parameters</partinfo>
 
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Latest revision as of 21:45, 18 September 2015

B0034-xylB

This composite represents the D-xylose dehydrogenase xylB plus the ribosomal binding site B0034.

Usage

This part is a composite of one coding gene and a strong RBS (BBa_B0034).

We used this part for our D-xylonic acid producing operon (BBa_K1602017)

  • xylB
  • RBS-B0034
https://static.igem.org/mediawiki/parts/5/58/TU_Darmstadt_plasmid_cardB0034-xylB.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]