Difference between revisions of "Part:BBa K1720002:Design"
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The hypoxia response element is a enhancer. So this part do not contains promoter,so that users can choose their own promoter for different purpose.When you use this part please add your own promoter.
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===Design Notes:===
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We cloned this basic part into a psb1c3 vector.We designed a hypoxia responsive CMV promotor by inserting a hypoxia responsive element （HRE） to CMV promotor.The hypoxia-responsive element is a minimalcis-regulatory element mediating transactivation by the hypoxia-inducible factor (HIF) in mammalian.So that this part only applies to mammalian organism.
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===References===
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1.Rational design of minimal hypoxia-inducible enhancers
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Stefan Kaluz*, Milota Kaluzová, Eric J. Stanbridge
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Department of Microbiology and Molecular Genetics, Medical Science I, B210, University of California at Irvine, College of Medicine, CA 92697-4025, USA

Latest revision as of 02:33, 18 September 2015


Design Notes:

We cloned this basic part into a psb1c3 vector.We designed a hypoxia responsive CMV promotor by inserting a hypoxia responsive element （HRE） to CMV promotor.The hypoxia-responsive element is a minimalcis-regulatory element mediating transactivation by the hypoxia-inducible factor (HIF) in mammalian.So that this part only applies to mammalian organism.

References

1.Rational design of minimal hypoxia-inducible enhancers Stefan Kaluz*, Milota Kaluzová, Eric J. Stanbridge Department of Microbiology and Molecular Genetics, Medical Science I, B210, University of California at Irvine, College of Medicine, CA 92697-4025, USA