Difference between revisions of "Part:BBa K1607010:Design"
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<partinfo>BBa_K1607010 short</partinfo> | <partinfo>BBa_K1607010 short</partinfo> | ||
<partinfo>BBa_K1607010 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1607010 SequenceAndFeatures</partinfo> | ||
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===Source=== | ===Source=== | ||
This coding sequence was synthesized by LCR assmebly using 36 oilgos | This coding sequence was synthesized by LCR assmebly using 36 oilgos | ||
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| − | + | ===Design note=== | |
| + | '''Isolation:''' | ||
After the LCR assembly, we isolated the CDS using these two primers(with BioBrick Prefix in the fwd primer and Suffix in the rev primer): | After the LCR assembly, we isolated the CDS using these two primers(with BioBrick Prefix in the fwd primer and Suffix in the rev primer): | ||
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fwd:GAATTCGCGGCCGCTTCTAGatgGGTTCTACTCAAGttt | fwd:GAATTCGCGGCCGCTTCTAGatgGGTTCTACTCAAGttt | ||
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rev:TACTAGTAGCGGCCGCTGCAGAGATTGACAATCTTCAGAAGAT | rev:TACTAGTAGCGGCCGCTGCAGAGATTGACAATCTTCAGAAGAT | ||
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| + | '''The termination codon:''' | ||
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| + | Since it was originally designed for 3'his-tag vectors, this coding sequence does not have a termination codon. | ||
===References=== | ===References=== | ||
[1]Zhou H, Zha Z, Liu Y, et al. Structural Insights into the Down-regulation of Overexpressed p185her2/neu Protein of Transformed Cells by the Antibody chA21[J]. Journal of Biological Chemistry, 2011, 286(36): 31676-31683. | [1]Zhou H, Zha Z, Liu Y, et al. Structural Insights into the Down-regulation of Overexpressed p185her2/neu Protein of Transformed Cells by the Antibody chA21[J]. Journal of Biological Chemistry, 2011, 286(36): 31676-31683. | ||
Latest revision as of 06:02, 24 August 2015
The coding sequence of the SCFV of anti-p185her2/neu antibody chA21
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 370
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Source
This coding sequence was synthesized by LCR assmebly using 36 oilgos
Design note
Isolation:
After the LCR assembly, we isolated the CDS using these two primers(with BioBrick Prefix in the fwd primer and Suffix in the rev primer):
fwd:GAATTCGCGGCCGCTTCTAGatgGGTTCTACTCAAGttt
rev:TACTAGTAGCGGCCGCTGCAGAGATTGACAATCTTCAGAAGAT
The termination codon:
Since it was originally designed for 3'his-tag vectors, this coding sequence does not have a termination codon.
References
[1]Zhou H, Zha Z, Liu Y, et al. Structural Insights into the Down-regulation of Overexpressed p185her2/neu Protein of Transformed Cells by the Antibody chA21[J]. Journal of Biological Chemistry, 2011, 286(36): 31676-31683.