Difference between revisions of "Part:BBa K1758100:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | We derived this sequence from the literature, searching for the best suited enhancing sequence for ''in vitro'' translation. Our main literature sources were [http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989], [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161345/ Shin and Noireaux 2010], [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128707/ Zawada et al. 2011] and [http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]. | + | We derived this sequence from the literature, searching for the best suited enhancing sequence for ''in vitro'' translation. Our main literature sources were [http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989], [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161345/ Shin and Noireaux 2010], [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128707/ Zawada et al. 2011], [http://www.ncbi.nlm.nih.gov/pubmed/23654270 Lentini et al. 2013] and [http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]. |
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===Source=== | ===Source=== | ||
− | This part was created by applying Gibson primers with suitable | + | This part was created by applying Gibson primers with suitable overhangs on pSB1C3. |
===References=== | ===References=== |
Latest revision as of 12:36, 16 October 2019
Translation enhancing 5-UTR containing g10-L RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We derived this sequence from the literature, searching for the best suited enhancing sequence for in vitro translation. Our main literature sources were [http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989], [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161345/ Shin and Noireaux 2010], [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128707/ Zawada et al. 2011], [http://www.ncbi.nlm.nih.gov/pubmed/23654270 Lentini et al. 2013] and [http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013].
Source
This part was created by applying Gibson primers with suitable overhangs on pSB1C3.