Difference between revisions of "Part:BBa K1604020:Design"
 
(5 intermediate revisions by 2 users not shown)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Standard assembly between BBA_K731201 and a PCR amplification of BBa_K274200.  
+
Standard assembly between <html><a href="https://parts.igem.org/Part:BBa_K731201">BBa_K731201</a></html> and a PCR amplification of <html><a href="https://parts.igem.org/Part:BBa_K274200">Ba_K274200</a></html>.  
−
BBa_K274200 was extracted by the 2012 distribution kit in pSB1A2 and it was amplified by PCR to restore a missing SpEI site.
+
  
 +
<html><a href="https://parts.igem.org/Part:BBa_K274200">Ba_K274200</a></html> was extracted by the 2012 distribution kit in pSB1A2
  
 +
NOTE
 +
This device was built using a part from the Cambridge 2009 team and the arabinose promoter from Trento 2012.  Be careful when operating this part, because the SpEI  site in the suffix is missing. The part from Cambridge 2009 had this mistake and so the error was introduced in our part. We realized that after sequencing. Other than that the device work well.
  
 
===Source===
 
===Source===
−
Arabinose inducible araC-pBAD promoter come from E. coli genome.
+
Arabinose inducible araC-pBAD promoter come from <i>E. coli</i> genome.
−
Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora).
+
Beta carotene gene coding sequences come from ''<i>Pantoea ananatis</i> '' (formerly <i>Erwinia uredovora</i> ) genome.
−
 
+
−
 
+
  
 
===References===
 
===References===

Latest revision as of 12:05, 16 September 2015

araC-pBAD + beta-carotene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 3185
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2721
    Illegal NgoMIV site found at 2851
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1936
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Standard assembly between BBa_K731201 and a PCR amplification of Ba_K274200.

Ba_K274200 was extracted by the 2012 distribution kit in pSB1A2

NOTE This device was built using a part from the Cambridge 2009 team and the arabinose promoter from Trento 2012. Be careful when operating this part, because the SpEI site in the suffix is missing. The part from Cambridge 2009 had this mistake and so the error was introduced in our part. We realized that after sequencing. Other than that the device work well.

Source

Arabinose inducible araC-pBAD promoter come from E. coli genome. Beta carotene gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora ) genome.

References