Difference between revisions of "Part:BBa K1758120:Design"

 
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===Design Notes===
 
===Design Notes===
 
'''Amplification with Gibson-primer:'''  
 
'''Amplification with Gibson-primer:'''  
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*Amplification of regulator of ribonuclease activity A (''RraA''):
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*Amplification of regulator of ribonuclease activity A (RraA):
  
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'''Forward primer RraA'''
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'''Forward primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#RraA_fwd RraA_fwd]'''
  
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5'-ATGAAATACGATACTTCCGAGCTTTG-3'
 
  
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'''Reverse primer RraA'''
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'''Reverse primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#RraA_rev RraA_rev]'''
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5'-TCATTCAATATCCAGCGGATCTTC-3'
 
  
 
*Amplification of the pSB1C3-Backbone:
 
*Amplification of the pSB1C3-Backbone:
  
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'''Forward primer pSB1C3 RraA(43 bp)'''
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'''Forward primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pBS1C3_RraA_pre pSB1C3_RraA_pre]'''
  
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5'-TCAGAAGATCCGCTGGATATTGAATGATACTAGTAGCGGCCGCTGCAG-3'
 
  
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'''Reverse primer pSB1C3 RraA(43 bp)'''
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'''Reverse primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pBS1C3_RraA_suf pSB1C3_RraA_suf]'''
  
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5'-TTCTTCCAGTTGTTCCGGGTTTGCCATCTCTAGAAGCGGCCGCGAATTCC-3'
 
  
 
'''Ligation via [http://2015.igem.org/Team:Bielefeld-CeBiTec/Protocols Gibson-Assembly].'''
 
'''Ligation via [http://2015.igem.org/Team:Bielefeld-CeBiTec/Protocols Gibson-Assembly].'''
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−
 
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===Source===
 
===Source===

Latest revision as of 23:40, 21 August 2015


RNase E regulating protein RraA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amplification with Gibson-primer:

  • Amplification of regulator of ribonuclease activity A (RraA):

Forward primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#RraA_fwd RraA_fwd]


Reverse primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#RraA_rev RraA_rev]


  • Amplification of the pSB1C3-Backbone:

Forward primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pBS1C3_RraA_pre pSB1C3_RraA_pre]


Reverse primer [http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pBS1C3_RraA_suf pSB1C3_RraA_suf]


Ligation via [http://2015.igem.org/Team:Bielefeld-CeBiTec/Protocols Gibson-Assembly].

Source

RraA was obtained via PCR on E. coli K12 genomic DNA.

References