Difference between revisions of "Part:BBa K1659100:Design"
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− | ===Design Notes=== | + | ===Design Notes and Sources=== |
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− | + | A Hisx6 tag is added at the C-terminus for ease of protein purification using metal-affinity chromatography. | |
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+ | We obtained the peptide sequence for MccS from Zschüttig et al's original publication and converted it into a nucleotide sequence using [https://www.idtdna.com/CodonOpt IDT's Codon Optimizer], optimizing based on ''E. coli'' K-12's codon table [1]. | ||
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===References=== | ===References=== | ||
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[1] Zschüttig, A., Zimmermann, K., Blom, J., Goesmann, A., Pöhlmann, C., & Gunzer, F. (2012). Identification and characterization of microcin S, a new antibacterial peptide produced by probiotic Escherichia coli G3/10. PLoS ONE, 7(3), 1–9. http://doi.org/10.1371/journal.pone.0033351 | [1] Zschüttig, A., Zimmermann, K., Blom, J., Goesmann, A., Pöhlmann, C., & Gunzer, F. (2012). Identification and characterization of microcin S, a new antibacterial peptide produced by probiotic Escherichia coli G3/10. PLoS ONE, 7(3), 1–9. http://doi.org/10.1371/journal.pone.0033351 |
Latest revision as of 16:23, 12 September 2015
Microcin S, a Class II microcin produced by probiotic E. coli G3/10
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes and Sources
A Hisx6 tag is added at the C-terminus for ease of protein purification using metal-affinity chromatography.
We obtained the peptide sequence for MccS from Zschüttig et al's original publication and converted it into a nucleotide sequence using IDT's Codon Optimizer, optimizing based on E. coli K-12's codon table [1].
References
[1] Zschüttig, A., Zimmermann, K., Blom, J., Goesmann, A., Pöhlmann, C., & Gunzer, F. (2012). Identification and characterization of microcin S, a new antibacterial peptide produced by probiotic Escherichia coli G3/10. PLoS ONE, 7(3), 1–9. http://doi.org/10.1371/journal.pone.0033351