Difference between revisions of "Part:BBa K1813028"
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<partinfo>BBa_K1813028 short</partinfo> | <partinfo>BBa_K1813028 short</partinfo> | ||
− | LacI | + | A construct used to achieve the bacterial expression of <i>A. gambiae</i> NADPH p450 reductase (<html><b><a href="https://parts.igem.org/Part:BBa_K1813010">BBa_K1813010</b></a></html>) under regulative authority of the LacI repressor. |
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===Functional Parameters=== | ===Functional Parameters=== | ||
− | <partinfo> | + | <partinfo>BBa_K1813024 parameters</partinfo> |
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+ | <h2> Background </h2> | ||
+ | Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of <i>A. gambiae</i> NADPH p450 reductase without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host. |
Latest revision as of 08:43, 18 September 2015
LacI Reversed AgamNADPH Reductase
A construct used to achieve the bacterial expression of A. gambiae NADPH p450 reductase (BBa_K1813010) under regulative authority of the LacI repressor.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 105
Illegal BamHI site found at 1887 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1685
Illegal AgeI site found at 2867
Illegal AgeI site found at 3023 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3383
Background
Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of A. gambiae NADPH p450 reductase without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host.