Difference between revisions of "Part:BBa K1813025"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1813025 short</partinfo>
 
<partinfo>BBa_K1813025 short</partinfo>
  
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LacI Reversed CYP6G1
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A construct used to achieve the bacterial expression of CYP6G1 (<html><b><a href="https://parts.igem.org/Part:BBa_K1813004">BBa_K1813004</b></a></html>) under the regulative authority of the LacI repressor.
  
  
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<!-- Uncomment this to enable Functional Parameter display  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1813025 parameters</partinfo>
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<partinfo>BBa_K1813024 parameters</partinfo>
 
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<h2> Background </h2>
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Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of CYP6G1 without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host.

Latest revision as of 08:44, 18 September 2015

CYP6G1 Expression Cassette with LacI Reversed

A construct used to achieve the bacterial expression of CYP6G1 (BBa_K1813004) under the regulative authority of the LacI repressor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2357


Background

Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of CYP6G1 without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host.