Difference between revisions of "Part:BBa K1628102"

 
 
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<partinfo>BBa_K1628102 short</partinfo>
 
<partinfo>BBa_K1628102 short</partinfo>
  
This part is consist of the following four sequences. 14bp between B&C +450bp coding sequence of pgsC +18bp between C&A +1149 coding sequence of pgsA.
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''pgsC'' and ''pgsA'' are two genes in ''pgsBCA'' operon. Protein PgsBCA is a membrane protein responsible for the synthesis of γ-PGA. Subunit PgsC is responsible for glutamic acid’s polymerization and subunit PgsA is responsible for the secretion of γ-PGA (showed in Figure 3).  
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[[File:pgsBCA1_NK.png]]
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Funtion of part pgsB and pgsCA were validated through fermentation experiment. Wild type ''Bacillus amyloliquefaciens'' NK-1 strain could produce 3-4g/L γ-PGA after 48 hours of fermentation. In previous work in our laboratory, we also tested heterologous expression of γ-PGA in ''E. coli''. We constructed different expression vectors containing ''pgsBCA'' genes and transformed them separately into ''E.coli'' JM109. The average production of γ-PGA in ''E. coli'' JM109 is around 0.5g/L.
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[[File:pgsBCA2_NK.png]]
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 08:19, 18 September 2015

pgsCA

pgsC and pgsA are two genes in pgsBCA operon. Protein PgsBCA is a membrane protein responsible for the synthesis of γ-PGA. Subunit PgsC is responsible for glutamic acid’s polymerization and subunit PgsA is responsible for the secretion of γ-PGA (showed in Figure 3).

PgsBCA1 NK.png

Funtion of part pgsB and pgsCA were validated through fermentation experiment. Wild type Bacillus amyloliquefaciens NK-1 strain could produce 3-4g/L γ-PGA after 48 hours of fermentation. In previous work in our laboratory, we also tested heterologous expression of γ-PGA in E. coli. We constructed different expression vectors containing pgsBCA genes and transformed them separately into E.coli JM109. The average production of γ-PGA in E. coli JM109 is around 0.5g/L.

PgsBCA2 NK.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]