Difference between revisions of "Part:BBa K1628101"

Latest revision as of 08:03, 18 September 2015

pgsB

pgsB is a gene responsible for γ-PGA synthesis in pgsBCA operon. Protein PgsBCA is a membrane protein and subunit PgsB’s main function is gathering substrate glutamic acid for γ-PGA synthesis (showed in Figure 1).

Funtion of part pgsB and pgsCA were validated through fermentation experiment. Wild type Bacillus amyloliquefaciens NK-1 strain could produce 3-4g/L γ-PGA after 48 hours of fermentation. In previous work in our laboratory, we also tested heterologous expression of γ-PGA in E. coli. We constructed different expression vectors containing pgsBCA genes and transformed them separately into E.coli JM109. The average production of γ-PGA in E. coli JM109 is around 0.5g/L.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1628101 short</partinfo>
-pgsB, first part of the coding sequence of gene pgsBCA.
+''pgsB'' is a gene responsible for γ-PGA synthesis in ''pgsBCA'' operon. Protein PgsBCA is a membrane protein and subunit PgsB’s main function is gathering substrate glutamic acid for γ-PGA synthesis (showed in Figure 1).
+[[File:pgsBCA1_NK.png]]
+Funtion of part pgsB and pgsCA were validated through fermentation experiment. Wild type ''Bacillus amyloliquefaciens'' NK-1 strain could produce 3-4g/L γ-PGA after 48 hours of fermentation. In previous work in our laboratory, we also tested heterologous expression of γ-PGA in ''E. coli''. We constructed different expression vectors containing ''pgsBCA'' genes and transformed them separately into ''E.coli'' JM109. The average production of γ-PGA in ''E. coli'' JM109 is around 0.5g/L.
+[[File:pgsBCA2_NK.png]]
 <!-- Add more about the biology of this part here