Difference between revisions of "Part:BBa K1628001"

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<partinfo>BBa_K1628001 short</partinfo>
 
<partinfo>BBa_K1628001 short</partinfo>
  
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Original promoter of pgsBCA. Weak.
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Promoter Pbca is the original promoter of ''pgsBCA'' operon. We have validate this part through promoter strength assay using ''bgaB'' (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to the result of promoter strength assay (showed in Figure 1), the strength of Pbca is very weak.
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[[File:promoter4_NK.png]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:56, 18 September 2015

Pbca

Promoter Pbca is the original promoter of pgsBCA operon. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to the result of promoter strength assay (showed in Figure 1), the strength of Pbca is very weak.

Promoter4 NK.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]