Difference between revisions of "Part:BBa K1813004"
(8 intermediate revisions by the same user not shown) | |||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1813004 short</partinfo> | <partinfo>BBa_K1813004 short</partinfo> | ||
CYP6G1 | CYP6G1 | ||
+ | |||
+ | CYP6G1 is a cytochrome p450 enzyme found in the common fruit fly, <i> Drosophila melanogaster</i>. Able to efficiently Hydroxylate imidacloprid at the 4th and 5th carbons, it is capable of bestowing protective properties to organisms that can express it. <br> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | CYP6G1 is a cytochrome p450 enzyme from <i>Drosophila melanogaste </i> capable of hydroxylating imidacloprid at the 4 and carbons. | |
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Line 17: | Line 18: | ||
<partinfo>BBa_K1813004 parameters</partinfo> | <partinfo>BBa_K1813004 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
+ | |||
+ | <h2>Background of <i>CYP6G1</i></h2> | ||
+ | As an enzyme capable of hydroxylating the 4 and 5 carbons of imidacloprid, it is capable of bestowing a significant protective effect to insects harboring this gene. It has an affinity to 6OH imidacloprid, allowing it to gnerate 4,5 OH imidacloprid [1], which has an ld50 of over 1 microgram vs 57 nanograms for imidacloprid [2]. Used in conjunction with CYP6CM1vQ, (BBa_K1813003) it can be extremely effective in bestowing imidacloprid resistance to an organism. <br> | ||
+ | This coding sequence was synthesized and is codon optimized for <i> E. coli</i>. It was originally identified in <i> Drosophila melanogaster</i>, the common fruitfly [1]. | ||
+ | This part is also used in<html><b> <a href="https://parts.igem.org/Part:BBa_K1613003"> BBa_K1613003</a></b></html> and <html><b> <a href="https://parts.igem.org/Part:BBa_K1813012"> BBa_K1813012</a></b></html> | ||
+ | |||
+ | |||
+ | |||
+ | <h2> References </h2> | ||
+ | |||
+ | [1] Joußen, Nicole, et al. "Metabolism of imidacloprid and DDT by P450 CYP6G1 expressed in cell cultures of Nicotiana tabacum suggests detoxification of these insecticides in Cyp6g1‐overexpressing strains of Drosophila melanogaster, leading to resistance." Pest management science 64.1 (2008): 65-73. <br> | ||
+ | |||
+ | [2]Suchail, S., Guez, D., & Belzunces, L. P. (2001). Discrepancy between acute and chronic toxicity induced by imidacloprid and its metabolites in Apis mellifera. Environmental toxicology and chemistry, 20(11), 2482-2486. |
Latest revision as of 07:35, 18 September 2015
CYP6G1
CYP6G1
CYP6G1 is a cytochrome p450 enzyme found in the common fruit fly, Drosophila melanogaster. Able to efficiently Hydroxylate imidacloprid at the 4th and 5th carbons, it is capable of bestowing protective properties to organisms that can express it.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 934
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 943
Background of CYP6G1
As an enzyme capable of hydroxylating the 4 and 5 carbons of imidacloprid, it is capable of bestowing a significant protective effect to insects harboring this gene. It has an affinity to 6OH imidacloprid, allowing it to gnerate 4,5 OH imidacloprid [1], which has an ld50 of over 1 microgram vs 57 nanograms for imidacloprid [2]. Used in conjunction with CYP6CM1vQ, (BBa_K1813003) it can be extremely effective in bestowing imidacloprid resistance to an organism.
This coding sequence was synthesized and is codon optimized for E. coli. It was originally identified in Drosophila melanogaster, the common fruitfly [1].
This part is also used in BBa_K1613003 and BBa_K1813012
References
[1] Joußen, Nicole, et al. "Metabolism of imidacloprid and DDT by P450 CYP6G1 expressed in cell cultures of Nicotiana tabacum suggests detoxification of these insecticides in Cyp6g1‐overexpressing strains of Drosophila melanogaster, leading to resistance." Pest management science 64.1 (2008): 65-73.
[2]Suchail, S., Guez, D., & Belzunces, L. P. (2001). Discrepancy between acute and chronic toxicity induced by imidacloprid and its metabolites in Apis mellifera. Environmental toxicology and chemistry, 20(11), 2482-2486.