Difference between revisions of "Part:BBa K1648001"
 
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Consist of six fragments from magnetosome island of ''Magnetospirillum gryphiswaldense'' genome.
 
Consist of six fragments from magnetosome island of ''Magnetospirillum gryphiswaldense'' genome.
  
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Due to potential difficulty in introducing the large magnetosome formation operons into ''Azotobacter vinelandii'', a "template" consists of flanking sequences from '''mamXY, mamGFDC and mms6 operons''' is designed for homologous recombination.
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Due to potential difficulty in introducing the large magnetosome formation operons into ''Azotobacter vinelandii'', a "template" consists of flanking sequences from '''mamXY, mamGFDC and mms6 operons''' is designed for '''homologous recombination'''.
  
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This construct is linearized and randomly integrated into ''A. vinelandii'', before full operon sequences to be transformed into ''A. vinelandii''.
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Part containing this template ([https://parts.igem.org/Part:BBa_K1648003 K1648003]) is introduced into ''A. vinelandii'' genome via '''random integration''' before full operon sequences to be transformed into ''A. vinelandii''.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 05:08, 20 September 2015

Construct Map for K1648001

Recombination Template for mamXY, mamGC and mms Operons

Consist of six fragments from magnetosome island of Magnetospirillum gryphiswaldense genome.

Due to potential difficulty in introducing the large magnetosome formation operons into Azotobacter vinelandii, a "template" consists of flanking sequences from mamXY, mamGFDC and mms6 operons is designed for homologous recombination.

Part containing this template (K1648003) is introduced into A. vinelandii genome via random integration before full operon sequences to be transformed into A. vinelandii.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1333
    Illegal BamHI site found at 432
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 603
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 875
    Illegal NgoMIV site found at 1283
    Illegal AgeI site found at 486
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 470
    Illegal BsaI site found at 569
    Illegal BsaI site found at 850