Difference between revisions of "Part:BBa K1648000:Design"

 
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===Design Notes===
 
===Design Notes===
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In order to form a homologous recombination template, we select 250 bp of each fragment and do the overlapping PCR ligate fragments together.
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In order to form a homologous recombination template, we selected 250 bp of 5' and 3' ends from '''mamHIEJKLMN''' and '''mamOPAQRBSTU''' (parts of '''mamAB operon''') as flanking sequences. Fragments are joined via overlapping PCR.
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[[File:K1648000 History.png|900px]]
  
 
===Source===
 
===Source===

Latest revision as of 02:04, 15 July 2015


Recombination Template for mamAB Operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 775
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 291
    Illegal AgeI site found at 810
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 536


Design Notes

In order to form a homologous recombination template, we selected 250 bp of 5' and 3' ends from mamHIEJKLMN and mamOPAQRBSTU (parts of mamAB operon) as flanking sequences. Fragments are joined via overlapping PCR.

K1648000 History.png

Source

Magnetospirillum gryphiswaldense MSR-1 v2, complete genome

References