Difference between revisions of "Part:BBa K1773005:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1773005 short</partinfo> | <partinfo>BBa_K1773005 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | + | Mutagenesis primers: | |
+ | |||
+ | Fw: cgcacatttcccgtggaatccactcttcctcaaaagg | ||
+ | |||
+ | Rev: ccttttgaggaagagtggattccacgggaaatgtgcg | ||
+ | |||
+ | |||
+ | Primers for Suffix and prefix insertion: | ||
+ | |||
+ | Fw: actgaattcgcggccgcttctagatgataactaaaacaacagtagtaaaagc | ||
+ | |||
+ | Rev: cggctgcagcggccgctactagtaattaattagaaatgtggaacggttg | ||
+ | |||
Latest revision as of 15:01, 10 September 2015
I-F type CRISPR-Cas Cascade complex
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1790
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2307
Design Notes
Mutagenesis primers:
Fw: cgcacatttcccgtggaatccactcttcctcaaaagg
Rev: ccttttgaggaagagtggattccacgggaaatgtgcg
Primers for Suffix and prefix insertion:
Fw: actgaattcgcggccgcttctagatgataactaaaacaacagtagtaaaagc
Rev: cggctgcagcggccgctactagtaattaattagaaatgtggaacggttg
Source
Aggregatibacter actinomycetemcomitans D-7S