Difference between revisions of "Part:BBa K1648048"

Latest revision as of 15:10, 25 September 2015

AvOprF induced by J13002

Construct Map for K1648048

OprF is a transmembrane porin which allows non-specific transmission. For constructing an efficient microbial fuel cell(MFC), we manipulated the expression of oprF. oprF allows electron carriers shuttle in and out of the bacteria, the electron generator. The electrons generated by the bacteria are taken up by the incoming carrier. Reduced electron carriers transport the electrons out of the bacteria to the electrode. BBa_K1648048 provides constitutive transcription of oprF of Azotobacter vinelandii.

An experiment was carried out for the comparison between the transformed bacteria with BBa_K1648048 for oprF from Azotobacter vinelandii BBa_K1648049 for mutated oprF from Azotobacter vinelandii and BBa_K1648050 for BBa_K1172501 from Germany iGEM team. For comparison,an identical promoter, J13002, which is a constitutive promoter, is chosen to add before the gene in psb1C3 for constitutive expression of different OprF in bacteria.

Double digestion of K1648048: L: DNA ladder. Lane 1-3: Recombination Template for J13002-oprF(BBa_K1648048) with double digestion cut at EcoR1 and PstI sites, with single digestion at Pst1 site, without digestion.

Comparison between the reduction of methylene blue in tetRpro-oprF and tetRpro-oprF* transformed E.coil.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 555

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1648048 short</partinfo>
-hoxKGZ operon from ''Azotobacter vinelandii''. Encodes structural subunits of membrane-bound hydrogenase (MBH).
+[[File:K1648048_Map.png|350px|thumb|right|Construct Map for K1648048]]
+<div style="text-align:justify; text-justify:inter-ideograph">
+OprF is a transmembrane porin which allows non-specific transmission.  For constructing an efficient microbial fuel cell(MFC), we manipulated the expression of oprF. oprF allows electron carriers shuttle in and out of the bacteria, the electron generator. The electrons generated by the bacteria are taken up by the incoming carrier. Reduced electron carriers transport the electrons out of the bacteria to the electrode. '''BBa_K1648048 provides constitutive transcription of oprF of ''Azotobacter vinelandii''.'''
+An experiment was carried out for the comparison between the transformed bacteria with [https://parts.igem.org/Part:BBa_K1648048 BBa_K1648048] for oprF from Azotobacter vinelandii [https://parts.igem.org/Part:BBa_K1648049 BBa_K1648049] for mutated oprF from Azotobacter vinelandii and [https://parts.igem.org/Part:BBa_K1648050 BBa_K1648050] for [https://parts.igem.org/Part:BBa_K1172501 BBa_K1172501] from Germany iGEM team. For comparison,an identical promoter, J13002, which is a constitutive promoter, is chosen to add before the gene in psb1C3 for constitutive expression of different OprF in bacteria.
+[[File:K1648048_dd.png|350px|thumb|right|Double digestion of K1648048:  L: DNA ladder. Lane 1-3: Recombination Template for J13002-oprF(BBa_K1648048) with double digestion cut at EcoR1 and PstI sites, with single digestion at Pst1 site, without digestion.]]
+[[File:data.png|350px|thumb|centre|Comparison between the reduction of methylene blue in tetRpro-oprF and tetRpro-oprF* transformed E.coil.]]
 <!-- Add more about the biology of this part here