Difference between revisions of "Part:BBa K1648045:Design"

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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K1648000 short</partinfo>
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<partinfo>BBa_K1648045 short</partinfo>
  
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<partinfo>BBa_K1648000 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1648045 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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PCR with BBa_K1314016 as template using the following primers:
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PCR with Azotobacter vinelandii as template using the following primers:
  
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>SH3 Domain F
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Forward primer:
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GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGCAGAGTATGTGCGGGC
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GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAACTGAAAAACACCTTGGGCG
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>SH3 Domain R
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GTTTCTTCCTGCAGCGGCCGCTACTAGTATACTTCTCCACGTAAGGGACAGG
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===Source===
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Reverse primer:
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GTTTCTTCCTGCAGCGGCCGCTACTAGTATTACTGGGCTTGGGCTTCCAC
  
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Synthesis.
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Insert oprF PCR product into psB1C3
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===Source===
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The oprF gene was obtained by PCR from the Azotobacter vinelandii strain DJ genome.
  
 
===References===
 
===References===

Latest revision as of 08:25, 17 September 2015

AvOprF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 475


Design Notes

PCR with Azotobacter vinelandii as template using the following primers:

Forward primer: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAACTGAAAAACACCTTGGGCG

Reverse primer: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTACTGGGCTTGGGCTTCCAC

Insert oprF PCR product into psB1C3

Source

The oprF gene was obtained by PCR from the Azotobacter vinelandii strain DJ genome.

References