Difference between revisions of "Part:BBa K1648045"
 
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<partinfo>BBa_K1648000 short</partinfo>
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<partinfo>BBa_K1648045 short</partinfo>
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[[File:K1648045_Map.png|350px|thumb|right|Construct Map for K1648045]]
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OprF is a transmembrane porin which allows non-specific transmission.  For constructing an efficient microbial fuel cell(MFC), we manipulated the expression of oprF. oprF allows electron carriers shuttle in and out of the bacteria, the electron generator. The electrons generated by the bacteria are taken up by the incoming carrier. Reduced electron carriers transport the electrons out of the bacteria to the electrode. '''BBa_K1648045 is the basic part coding from Azotobacter vinelandii.'''
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[[File:K1648045_pcr.png|250px|thumb|left|PCR product of K1648045:  The photo of 1% agarose gel electrophoresis. L: DNA ladder. Lane 1: PCR product of oprF encoding from the Azotobacter vinelandii strain DJ genome.
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[[File:K1648045_dd.png|350px|thumb|center|Double digestion of K1648045: L: DNA ladder. Lane 1-3: Recombination Template for pSB1C3-oprF(BBa_K1648045) with double digestion cut at EcoR1 and PstI sites, with single digestion at Pst1 site, without digestion.
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Interacts with SH3 ligand (DNA: 5'-CCCCCTCCAGCGCTGCCGCCCAAACGTCGCCGG-3'; Protein: N-PPPALPPKRRR-C). Allows protein-protein interaction for those tagged with SH3 Domain and Ligand. Sequence derived from BBa_K771107.
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
 
===Functional Parameters===
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Latest revision as of 05:10, 20 September 2015

AvOprF

Construct Map for K1648045

OprF is a transmembrane porin which allows non-specific transmission. For constructing an efficient microbial fuel cell(MFC), we manipulated the expression of oprF. oprF allows electron carriers shuttle in and out of the bacteria, the electron generator. The electrons generated by the bacteria are taken up by the incoming carrier. Reduced electron carriers transport the electrons out of the bacteria to the electrode. BBa_K1648045 is the basic part coding from Azotobacter vinelandii.










PCR product of K1648045: The photo of 1% agarose gel electrophoresis. L: DNA ladder. Lane 1: PCR product of oprF encoding from the Azotobacter vinelandii strain DJ genome.
Double digestion of K1648045: L: DNA ladder. Lane 1-3: Recombination Template for pSB1C3-oprF(BBa_K1648045) with double digestion cut at EcoR1 and PstI sites, with single digestion at Pst1 site, without digestion.





Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 475