Difference between revisions of "Part:BBa K1638004:Design"

 
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===Design Notes===
 
===Design Notes===
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Addition of a protein coding domain to the suffix by Standard Assembly RFC[10] creates a scar-site. As the scarsite encodes a stop codon (uacuag), this will leave the fused protein coding domain untranslated.  
  
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Instead we recommend the following assembly method:
  
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1) Design primers for amplification of the C-terminal fusion protein with BamHI resitriction site included in the forward primer. The primers must also include prefix and suffix.
  
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===Source===
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1.2) Forward primer: 5'-ATATGGATCCNNN...NNN-3'
  
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1.3) Reverse primer: 5'-ATATCTGCAGCGGCCGCTACTAGTANNN..NNN-3'
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2) Amplify through PCR with designed primers
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3) Digest PCR-product and pSB1C3-T18 with BamHI and PstI.
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4) Ligate the two digested product.
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===Source===
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pUT18C
  
 
===References===
 
===References===

Latest revision as of 10:49, 23 July 2015


T18 domain of CyaA from Bordetella pertussis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 550
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 91
    Illegal NgoMIV site found at 501
    Illegal AgeI site found at 307
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Addition of a protein coding domain to the suffix by Standard Assembly RFC[10] creates a scar-site. As the scarsite encodes a stop codon (uacuag), this will leave the fused protein coding domain untranslated.

Instead we recommend the following assembly method:

1) Design primers for amplification of the C-terminal fusion protein with BamHI resitriction site included in the forward primer. The primers must also include prefix and suffix.

1.2) Forward primer: 5'-ATATGGATCCNNN...NNN-3'

1.3) Reverse primer: 5'-ATATCTGCAGCGGCCGCTACTAGTANNN..NNN-3'

2) Amplify through PCR with designed primers

3) Digest PCR-product and pSB1C3-T18 with BamHI and PstI.

4) Ligate the two digested product.

Source

pUT18C

References