Difference between revisions of "Help:Submission Kit"

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[[Category:Distribution Kit]][[Category:Part_Submission]]
 
{{HelpPage/GridCSS}}
 
{{HelpPage/GridCSS}}
 
{{HelpPage/MainLinks}}
 
{{HelpPage/MainLinks}}
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{{HelpPage/Updates/SampleSubmission}}
 
{{HelpPage/SendParts}}
 
{{HelpPage/SendParts}}
  
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For iGEM 2015, we are standardizing the part submission process.
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==Submission Format==
  
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As in previous years, parts must be '''BioBrick compatible''' and in '''pSB1C3'''. Additionally, all part submissions must be shipped as '''dried DNA''', in '''96 well plate format''', and covered with '''adhesive foil'''.
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<div style="font-size: 12pt">
  
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We've provided a submission kit with all of the materials you'll need to ship your parts.  
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* (3) 96-well skirted PCR plates
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<b>Additionally, all part submissions must be:</b>
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* (3) universal plate lids
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[[File:Sealed_Submission_Plate.jpg|right|300px]]
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* (5) adhesive foils
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*miniprepped plasmid DNA (over 250ng) '''dried down.''' 10ul of 25ng/ul for a total of 250ng works well.
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* bubble wrap
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*in '''96 well plate format''' (label your plate with your team name and submission number)
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* a box with the iGEM HQ address, for shipping your submission
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*plate sealed with '''adhesive foil'''
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**the adhesive foil should make full contact with the wells of the plate (see picture)
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*sealed plate covered with a '''plastic lid'''
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*shipped with a '''tracking number'''
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*shipped and labelled as '''DNA''', not hidden or disguised
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**DNA (non-hazardous, non-regulated, non-infectious, for research purposes only)
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----
  
  
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===Sample DNA Quantity===
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----
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* 250ng minimum of purified plasmid DNA (your part in pSB1C3)
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===Submission DNA Preparation===
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* 250ng minimum of purified plasmid DNA (your BioBrick compatible part in [[Help:Shipping|pSB1C3]])
 +
** 10ul of a 25ng/ul miniprep will work well
 
* DNA must be '''dried down''' before shipping
 
* DNA must be '''dried down''' before shipping
  
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We recommend drying down the plate in your lab's laminar flow hood.  
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We recommend drying down the plate in your lab's laminar flow hood (you can also leave your plate on the lab bench to air-dry, covered with the plastic lid).
 +
[[File:Sealed_Submission_Plate.jpg|right|200px]]
 
# Sterilize the hood surface with 70% Ethanol
 
# Sterilize the hood surface with 70% Ethanol
 
# Cover the 96 well plate with the provided plate lid (not with the adhesive foil)
 
# Cover the 96 well plate with the provided plate lid (not with the adhesive foil)
 
# Place the plate in the hood.  
 
# Place the plate in the hood.  
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# With sufficient airflow, drying down 10ul (25ng/ul) of purified plasmid DNA in the plate should take four hours.
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# With sufficient airflow, drying down 10ul of 25ng/ul (250ng total) of purified plasmid DNA in the plate should take 4-6 hours (or overnight).
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# Once all wells have dried, cover the plate with the provided adhesive foil.
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# Once all wells have dried, cover the '''plate wells''' with the provided adhesive foil.
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##The adhesive foil should make full contact with the wells of the plate (see picture). When properly sealed you should be able to see the impression of every well.
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# Cover the sealed plate with a plastic lid
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# Label your plate
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 +
 
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----
  
  
 
===Plate Labelling===
 
===Plate Labelling===
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Make sure to label each plate with the appropriate submission number.
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Make sure to label each plate with your group (team/lab) name and the appropriate submission number. Use a lab marker and write your team name and submission number on the side of the 96w plate.
  
 
Use one plate per a submission order. '''Example:''' If you are submitting 104 parts, create one submission order for 96 parts and another submission order for 8 parts. Label the plates with the correct submission order.
 
Use one plate per a submission order. '''Example:''' If you are submitting 104 parts, create one submission order for 96 parts and another submission order for 8 parts. Label the plates with the correct submission order.
 +
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===Note: When filling shipment info please make sure to declare and label the package as "DNA (non-hazardous, non-regulated, non-infectious, for research purposes only)."===
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 +
 +
----
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 +
===Packaging your submission for shipping===
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<html><p>Place your submission in an appropriately sized shipping container, such as a cardboard box, along with the printout of the submission form. If you use cushioning material inside the container, limit cushioning material to foam peanuts, bubble wrap, packaging paper, or similar.</p>
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<p><strong>Note:</strong> Part submissions packaged in materials other than the above will be rejected and these part submissions will not be taken into consideration for medals or awards. It is important that you not hide your submissions in an effort to conceal them from customs, because they will be seized.</p>
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<p><img src="https://static.igem.org/mediawiki/parts/b/b8/Bad_submission_packaging.png"></p></html>
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 +
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----
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===Archive===
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[[Help:Archive/Formats|Archived Submission Formats Page]]

Latest revision as of 18:38, 1 June 2020

Sample submission of parts is no longer a requirement for the iGEM competition. Also, iGEM HQ can no longer accept shipment of samples. Please do not ship any samples to our offices. Information in regards to shipping of samples to iGEM HQ has been kept solely for archival and consistency purposes.

Parts must still be documented on the Registry and follow the guidelines/requirements of the iGEM competition.

DNA Submission

Sample submission of parts is no longer a requirement for the iGEM competition.

Requirements

Here's how part samples are sent to the Registry

Why pSB1C3?

Why do samples need to be shipped in pSB1C3?

Detailed Instructions

Instructions on how to fill out the Registry's submission form.

Submission Prep

Registry groups must follow iGEM's submission prep guidelines.

Have questions on requirements or shipping part samples? Check the FAQ or contact us at hq (at) igem . org .

Submission Format


Additionally, all part submissions must be:

Sealed Submission Plate.jpg
  • miniprepped plasmid DNA (over 250ng) dried down. 10ul of 25ng/ul for a total of 250ng works well.
  • in 96 well plate format (label your plate with your team name and submission number)
  • plate sealed with adhesive foil
    • the adhesive foil should make full contact with the wells of the plate (see picture)
  • sealed plate covered with a plastic lid
  • shipped with a tracking number
  • shipped and labelled as DNA, not hidden or disguised
    • DNA (non-hazardous, non-regulated, non-infectious, for research purposes only)



Sample Layout

Make sure to add your samples in the correct layout:

  • Your first sample (s1) should be dispensed into well 1A of your submission plate.
  • Your second sample (s2) should be dispensed into well 1B of the same submission plate.

Use one plate per a submission order. Example: If you are submitting 104 parts, create one submission order for 96 parts and another submission order for 8 parts. Label the plates with the correct submission order.


Submission DNA Preparation

  • 250ng minimum of purified plasmid DNA (your BioBrick compatible part in pSB1C3)
    • 10ul of a 25ng/ul miniprep will work well
  • DNA must be dried down before shipping

We recommend drying down the plate in your lab's laminar flow hood (you can also leave your plate on the lab bench to air-dry, covered with the plastic lid).

Sealed Submission Plate.jpg
  1. Sterilize the hood surface with 70% Ethanol
  2. Cover the 96 well plate with the provided plate lid (not with the adhesive foil)
  3. Place the plate in the hood.
  4. With sufficient airflow, drying down 10ul of 25ng/ul (250ng total) of purified plasmid DNA in the plate should take 4-6 hours (or overnight).
  5. Once all wells have dried, cover the plate wells with the provided adhesive foil.
    1. The adhesive foil should make full contact with the wells of the plate (see picture). When properly sealed you should be able to see the impression of every well.
  6. Cover the sealed plate with a plastic lid
  7. Label your plate



Plate Labelling

Make sure to label each plate with your group (team/lab) name and the appropriate submission number. Use a lab marker and write your team name and submission number on the side of the 96w plate.

Use one plate per a submission order. Example: If you are submitting 104 parts, create one submission order for 96 parts and another submission order for 8 parts. Label the plates with the correct submission order.

Note: When filling shipment info please make sure to declare and label the package as "DNA (non-hazardous, non-regulated, non-infectious, for research purposes only)."

Packaging your submission for shipping

Place your submission in an appropriately sized shipping container, such as a cardboard box, along with the printout of the submission form. If you use cushioning material inside the container, limit cushioning material to foam peanuts, bubble wrap, packaging paper, or similar.

Note: Part submissions packaged in materials other than the above will be rejected and these part submissions will not be taken into consideration for medals or awards. It is important that you not hide your submissions in an effort to conceal them from customs, because they will be seized.



Archive

Archived Submission Formats Page