Difference between revisions of "Part:BBa J23095"
 
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<partinfo>BBa_J23095 short</partinfo>
 
<partinfo>BBa_J23095 short</partinfo>
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this is a ser2 derived tRNA key with an addressive sequence 27 bp long and whose 5'-most duplex is -28 relative to the start codon.[[Template:Berk2006iGEM-J23094-96Notes |.]]
  
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this is a ser2 derived tRNA key with an addressive sequence that anneals 5 bases past the 5' most part of j23077 lock's rbs' while still completely covering the rbs'. the 3' most base on the lock's rbs' will be called base (0). so key(0)'s 3'-most binding occurs at base (0) on the lock. key(n)'s 3' most binding occurs at base n. rbs' is the complement of the RBS in the lock's hairpin.
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{{Berk2006iGEM-J23094-96Notes}}
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 22:19, 21 November 2006

Ser2 tRNA key(-28) this is a ser2 derived tRNA key with an addressive sequence 27 bp long and whose 5'-most duplex is -28 relative to the start codon..

Berk2006iGEM-J23094toJ23096.gif

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 31
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]