Difference between revisions of "Part:BBa K1755003"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1755003 short</partinfo>
 
<partinfo>BBa_K1755003 short</partinfo>
  
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This part was a constitutive expression element for ribB.
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https://parts.igem.org/Part:BBa_K1755001#references
  
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  B0034+ribB+B0015
 
  
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<!-- Add more about the biology of this part here
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[[File:K1755003SHIYANTU.png]]
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===Usage and Biology===
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<!-- -->
 
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K1755003 SequenceAndFeatures</partinfo>
 
  
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This part consisting of B0032 and B0014 which were characterized as
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ribB coding sequence and double terminators.
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===Usage in MFC===
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In our project, we havea applied kinds of promoters
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which are sensitive to the concentration of copper heavy metal ions such as Cu2+ Hg2+ and Cr6+ to regulate the expression of protein ribB. [[File:HESHI.png]]
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The existence of ribB will enhance the production of riboflavin.
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Moreover, riboflavin, as the electron carrier, could improve the electricity producing performance of the Microbial Fuel Cells (MFCs).
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So in this way, we successfully correlated the concentration of heavy metal ions together with the output voltage of the MFC.
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===Usage and Biology===
  
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<!-- Uncomment this to enable Functional Parameter display
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When ribB coding sequence had RBS and terminator,we could regulate the expression of protein ribB with different promoters.
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===Functional Parameters===
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<partinfo>BBa_K1755003 parameters</partinfo>
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Latest revision as of 08:14, 28 August 2015

strong RBS + K1755002

This part was a constitutive expression element for ribB. https://parts.igem.org/Part:BBa_K1755001#references


K1755003SHIYANTU.png


This part consisting of B0032 and B0014 which were characterized as

ribB coding sequence and double terminators.

Usage in MFC

In our project, we havea applied kinds of promoters

which are sensitive to the concentration of copper heavy metal ions such as Cu2+ Hg2+ and Cr6+ to regulate the expression of protein ribB. HESHI.png

The existence of ribB will enhance the production of riboflavin.

Moreover, riboflavin, as the electron carrier, could improve the electricity producing performance of the Microbial Fuel Cells (MFCs).

So in this way, we successfully correlated the concentration of heavy metal ions together with the output voltage of the MFC.

Usage and Biology

When ribB coding sequence had RBS and terminator,we could regulate the expression of protein ribB with different promoters.