Difference between revisions of "Part:BBa J119377:Design"

 
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===Design Notes===
 
===Design Notes===
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The consensus sequence from De Mey et al. shown below was used to generate a promoter that has a simple sequence by using W=A, N=G, R=G, and D=G, but avoiding runs of 4 or more Gs or Cs.  The resulting promoter was named Psimp1.  
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The consensus sequence from De Mey et al. shown below was used to generate a promoter that has a simple sequence, by using W=T, N=C, R=A, and D=C, but avoiding runs of 4 or more Gs or Cs.  The resulting promoter was named Psimp1.  
  
 
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[[File:De_Mey_2007_Figure_1.jpg|750px|]]
 
[[File:De_Mey_2007_Figure_1.jpg|750px|]]
 
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===Source===
 
===Source===
  
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Synthetic oligonucleotides cloned into pClone Red (BBa_J119137).
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Synthetic oligonucleotides cloned with BsaI Golden Gate Assembly into [https://parts.igem.org/Part:BBa_J119137 pClone Red ](BBa_J119137).
  
 
===References===
 
===References===
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De Mey M, Maertens J, Lequeux GJ, Soetaiert WK, Vandamme EJ (2007)  Construction and model-based analysis of a promoter library for E. coli: an indispensable tool for metabolic engineering. MC Biotechnol 7:34.

Latest revision as of 13:34, 27 March 2015


Psimp2 Promoter with Four Consensus Variants


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The consensus sequence from De Mey et al. shown below was used to generate a promoter that has a simple sequence, by using W=T, N=C, R=A, and D=C, but avoiding runs of 4 or more Gs or Cs. The resulting promoter was named Psimp1.

De Mey 2007 Figure 1.jpg

Source

Synthetic oligonucleotides cloned with BsaI Golden Gate Assembly into pClone Red (BBa_J119137).

References

De Mey M, Maertens J, Lequeux GJ, Soetaiert WK, Vandamme EJ (2007) Construction and model-based analysis of a promoter library for E. coli: an indispensable tool for metabolic engineering. MC Biotechnol 7:34.