Difference between revisions of "Part:BBa M36019:Experience"
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From fluorescence assay conducted, we found that the sensor failed to perform its intended function (detecting lactic acid). Fluorescence/OD measurement remained constant across lactic acid concentrations on a log scale from 0 to 0.1M. We suspect that it is because E. coli themselves regulate lactate in the environment, hence over time the lactate concentration equilibrates to a constant concentration across all experimental set ups. | From fluorescence assay conducted, we found that the sensor failed to perform its intended function (detecting lactic acid). Fluorescence/OD measurement remained constant across lactic acid concentrations on a log scale from 0 to 0.1M. We suspect that it is because E. coli themselves regulate lactate in the environment, hence over time the lactate concentration equilibrates to a constant concentration across all experimental set ups. | ||
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- Barcode # 0133010776, 0133010749, 0133024645, 0133009701, 0133009727 | - Barcode # 0133010776, 0133010749, 0133024645, 0133009701, 0133009727 | ||
Latest revision as of 23:38, 5 December 2014
This experience page is provided so that any user may enter their experience using this part.
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Applications of BBa_M36019
Stanford Location By: Melissa, Sydney, Rachel (BIOE 44 Fall 2014) Experience:
From fluorescence assay conducted, we found that the sensor failed to perform its intended function (detecting lactic acid). Fluorescence/OD measurement remained constant across lactic acid concentrations on a log scale from 0 to 0.1M. We suspect that it is because E. coli themselves regulate lactate in the environment, hence over time the lactate concentration equilibrates to a constant concentration across all experimental set ups.
- Barcode # 0133010776, 0133010749, 0133024645, 0133009701, 0133009727
- Plasmid name: somcrl1
- Antibiotic resistance: amp
- DNA 2.0 gene # 193463
- Organism expressed in: E. Coli
- Sensor for lactic acid
User Reviews
UNIQ3510bd866c001b2a-partinfo-00000000-QINU UNIQ3510bd866c001b2a-partinfo-00000001-QINU