Difference between revisions of "Part:BBa M36921:Experience"
(→‎Performance Data of BBa_M36921)
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
  
 
'''Stanford Location'''
 
'''Stanford Location'''
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Plasmid Name:  
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DNA2.0 Gene #:
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Plasmid Name: TL- Zinc Sensor.gdx
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DNA2.0 Gene #: 193536
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Organism: ''E. coli''
 
Organism: ''E. coli''
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Device Type: Sensor
 
Device Type: Sensor
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Barcode #'s of Glycerol Stocks:
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Box Label:  
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Barcode #'s of Glycerol Stocks: 0133010718
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Box Label: BioE44 S14 Box 1
  
  
 
===Applications of BBa_M36921===
 
===Applications of BBa_M36921===
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This part has not been used in any other composite parts.
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===Performance Data of BBa_M36921===
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Our first trial had very promising results, showing an increase in fluorescence with increasing Zn2+ concentrations for our engineered plasmid. Our negative control plasmid (pJ1201-03c) also gave a low constant baseline of fluorescence. Unfortunately, in our second trial, we found that our negative control showed a similar "increasing" trend as the pZn. To test the statistical significance of our data from the second trial, we used a student t-Test. Our t-Test was a two-tailed test of our pZn and pJ1201 data populations assuming unequal variances. This test gave us p-value outputs that we compared with the threshold significance value of five percent. Two p-values were calculated (one for the sixteen hour reading & one for the twenty-four hour reading) for Zn2+ concentrations of 1000, 500, 1.95, and 0 mg/L and these values were used to try to disprove the null hypothesis that the means of the data populations are equal. Because half of our p-values were under .05 (0.026478414, 0.012607462, 0.035300697, 0.012522604) and half were over .05 (0.180998572, 0.291867597, 0.094258161, 0.335410155), the null hypothesis can not be invalidated, which shows that the data between our control plasmid and our engineered plasmid is statistically insignificant. However, this trial does not necessarily discredit our hypothesis and the functionality of our sensor. More trials need to be conducted with controlled variables and a greater number of data points are needed per Zn2+ concentration for more accurate p-values.
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[[File:Zinc data.tiff]]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 21:37, 14 January 2015


Stanford Location

Plasmid Name: TL- Zinc Sensor.gdx

DNA2.0 Gene #: 193536

Organism: E. coli

Device Type: Sensor

Barcode #'s of Glycerol Stocks: 0133010718

Box Label: BioE44 S14 Box 1


Applications of BBa_M36921

This part has not been used in any other composite parts.

Performance Data of BBa_M36921

Our first trial had very promising results, showing an increase in fluorescence with increasing Zn2+ concentrations for our engineered plasmid. Our negative control plasmid (pJ1201-03c) also gave a low constant baseline of fluorescence. Unfortunately, in our second trial, we found that our negative control showed a similar "increasing" trend as the pZn. To test the statistical significance of our data from the second trial, we used a student t-Test. Our t-Test was a two-tailed test of our pZn and pJ1201 data populations assuming unequal variances. This test gave us p-value outputs that we compared with the threshold significance value of five percent. Two p-values were calculated (one for the sixteen hour reading & one for the twenty-four hour reading) for Zn2+ concentrations of 1000, 500, 1.95, and 0 mg/L and these values were used to try to disprove the null hypothesis that the means of the data populations are equal. Because half of our p-values were under .05 (0.026478414, 0.012607462, 0.035300697, 0.012522604) and half were over .05 (0.180998572, 0.291867597, 0.094258161, 0.335410155), the null hypothesis can not be invalidated, which shows that the data between our control plasmid and our engineered plasmid is statistically insignificant. However, this trial does not necessarily discredit our hypothesis and the functionality of our sensor. More trials need to be conducted with controlled variables and a greater number of data points are needed per Zn2+ concentration for more accurate p-values.

File:Zinc data.tiff

User Reviews

UNIQf1d0519739af4bd2-partinfo-00000000-QINU UNIQf1d0519739af4bd2-partinfo-00000001-QINU