Difference between revisions of "Part:BBa M36939:Experience"
 
(One intermediate revision by the same user not shown)
Line 59: Line 59:
 
Organism: E. coli,
 
Organism: E. coli,
 
Device Type: LCPUFA Actuator.
 
Device Type: LCPUFA Actuator.
−
 
  
 
Barcode#: 133024994,
 
Barcode#: 133024994,
Line 69: Line 68:
  
 
'''Performance Data:'''
 
'''Performance Data:'''
−
 
−
The entries in the same numerical position in each list are data for one sample.
 
−
----
 
  
 
'''Protein Presence Test: GFP Fluorescence Reading'''
 
'''Protein Presence Test: GFP Fluorescence Reading'''
  
 
Note: GFP Fluorescence Readings were not taken for this composite part but for its sister part BBa_M36938 (contains Comet GFP). We suspect that similar results would be obtained for this construct (excluding Comet GFP). This data is included simply to provide a full analysis of the mechanism.  
 
Note: GFP Fluorescence Readings were not taken for this composite part but for its sister part BBa_M36938 (contains Comet GFP). We suspect that similar results would be obtained for this construct (excluding Comet GFP). This data is included simply to provide a full analysis of the mechanism.  
−
----
 
  
−
''Condition'' GFP GFP GFP GFP Control Control Control Control Blank
+
[[File:GFPchart.gif]]
−
----
+
 
−
                                         
+
 
−
''OD600''    1.44  1.377  1.422  1.377  1.432  1.448  1.45    1.451  0.037
+
[[File:GFPgraph.gif]]
−
----
+
 
  
−
''GFP AFU'' 3537 4250 3725 3604 829 966 918 853 658
 
−
----
 
−
''Standardized OD600'' 1.403 1.34 1.385 1.34 1.395 1.411 1.413 1.414 0
 
−
----
 
−
'' Standardized GFP AFU'' 2852 3565 3040 2919 144 281 233 168 0
 
−
----
 
−
'' AFU/OD600'' 2032.78689 2660.44776 2194.94585 2178.35821 103.225806 199.149539 164.897381 118.811881
 
−
----
 
−
''GFP Average:'' 2266.63468 ''Control Average:'' 146.521152
 
−
----
 
−
p = 0.00036738 ---> p < 0.001
 
−
----
 
 
Conclusion: The target protein (enoyl-[acyl-carrier-protein] reductase) is synthesized within the E. coli cells. This result is statistically significant.
 
Conclusion: The target protein (enoyl-[acyl-carrier-protein] reductase) is synthesized within the E. coli cells. This result is statistically significant.
  
Line 105: Line 88:
 
----
 
----
  
−
''Standard(no GFP) pH Readings:''
+
[[File:pHchart.jpg]]
−
6.91
+
−
6.64
+
−
6.49
+
−
6.42
+
−
6.64
+
−
6.45
+
−
6.34
+
−
6.4
+
−
----
+
  
−
''Control pH Readings:''
+
[[File:phgraph.jpg]]
  
−
6.56
 
−
6.49
 
−
6.42
 
−
6.36
 
−
6.25
 
−
6.26
 
−
6.29
 
−
6.34
 
−
----
 
−
''Standard Average pH:'' 6.53625
 
−
----
 
−
''Control Average pH:'' 6.37125
 
−
----
 
−
 
−
p=0.011066469 ---> p < 0.05
 
−
----
 
 
Conclusion: Production of the target protein (enoyl-[acyl-carrier-protein] reductase) causes an increase in pH, as is to be expected. Therefore we conclude that the target protein is functional. This is statistically significant.
 
Conclusion: Production of the target protein (enoyl-[acyl-carrier-protein] reductase) causes an increase in pH, as is to be expected. Therefore we conclude that the target protein is functional. This is statistically significant.
  

Latest revision as of 07:12, 4 December 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_M36939

Stanford Location:

Box Name: BIOE44 S14

Glycerol Freezer Stocks:

Barcode#: 133010734, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.

Barcode#: 133009653, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.

Barcode#: 133026955, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.

Barcode#: 133024660, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.

Barcode#: 133024658, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.

Barcode#: 133024682, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.

Barcode#: 133024673, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.

Barcode#: 133024994, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.

Performance Data:

Protein Presence Test: GFP Fluorescence Reading

Note: GFP Fluorescence Readings were not taken for this composite part but for its sister part BBa_M36938 (contains Comet GFP). We suspect that similar results would be obtained for this construct (excluding Comet GFP). This data is included simply to provide a full analysis of the mechanism.

GFPchart.gif


GFPgraph.gif


Conclusion: The target protein (enoyl-[acyl-carrier-protein] reductase) is synthesized within the E. coli cells. This result is statistically significant.


Protein Functionality Test: pH Reading

Functional (enoyl-[acyl-carrier-protein] reductase) is expected to partake in a naturally occurring reaction within the E. coli cell. This reaction consumes hydrogen ions. Thus a functional protein is expected to increase the overall pH of the cell.

PHchart.jpg

Phgraph.jpg

Conclusion: Production of the target protein (enoyl-[acyl-carrier-protein] reductase) causes an increase in pH, as is to be expected. Therefore we conclude that the target protein is functional. This is statistically significant.

User Reviews

UNIQ6a1609d30cf8c938-partinfo-00000000-QINU UNIQ6a1609d30cf8c938-partinfo-00000001-QINU