Difference between revisions of "Part:BBa M36938:Experience"

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===Applications of BBa_M36938===
 
===Applications of BBa_M36938===
  
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Stanford Location:  
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'''Stanford Location:'''
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Box Name: BIOE44 S14
 
Box Name: BIOE44 S14
  
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Sample Names:
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Glycerol Freezer Stocks:
  
 
Barcode# :133011796,
 
Barcode# :133011796,
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Organism: E. coli,
 
Organism: E. coli,
 
Device Type: LCPUFA Actuator.
 
Device Type: LCPUFA Actuator.
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'''Performance Data:'''
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'''Protein Presence Test: GFP Fluorescence Reading'''
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[[File:GFPchart.gif]]
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[[File:GFPgraph.gif]]
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Conclusion: The target protein (enoyl-[acyl-carrier-protein] reductase) is synthesized within the E. coli cells. This result is statistically significant.
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'''Protein Functionality Test: pH Reading'''
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Functional (enoyl-[acyl-carrier-protein] reductase) is expected to partake in a naturally occurring reaction within the E. coli cell. This reaction consumes hydrogen ions. Thus a functional protein is expected to increase the overall pH of the cell.
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Note: pH Readings were not taken for this composite part but for its sister part BBa_M36939 (lacks Comet GFP). We suspect that similar results would be obtained for this construct (containing Comet GFP). This data is included simply to provide a full analysis of the mechanism and its potential.
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[[File:pHchart.jpg]]
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[[File:phgraph.jpg]]
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Conclusion: Production of the target protein (enoyl-[acyl-carrier-protein] reductase) causes an increase in pH, as is to be expected. Therefore we conclude that the target protein is functional. This is statistically significant.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 07:05, 4 December 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_M36938

Stanford Location:

Box Name: BIOE44 S14

Glycerol Freezer Stocks:

Barcode# :133011796, Plasmid Name: CME LCPUFA_Dasher, Resistance Type: amp, DNA 2.0 Gene #:193425, Organism: E. coli, Device Type: LCPUFA Actuator.

Barcode# : 133024687, Plasmid Name: CME LCPUFA_Dasher, Resistance Type: amp, DNA 2.0 Gene #:193425, Organism: E. coli, Device Type: LCPUFA Actuator.

Barcode# : 133010087, Plasmid Name: CME LCPUFA_Dasher, Resistance Type: amp, DNA 2.0 Gene #:193425, Organism: E. coli, Device Type: LCPUFA Actuator.

Barcode# : 133011361, Plasmid Name: CME LCPUFA_Dasher, Resistance Type: amp, DNA 2.0 Gene #:193425, Organism: E. coli, Device Type: LCPUFA Actuator.

Performance Data:

Protein Presence Test: GFP Fluorescence Reading

GFPchart.gif


GFPgraph.gif


Conclusion: The target protein (enoyl-[acyl-carrier-protein] reductase) is synthesized within the E. coli cells. This result is statistically significant.


Protein Functionality Test: pH Reading

Functional (enoyl-[acyl-carrier-protein] reductase) is expected to partake in a naturally occurring reaction within the E. coli cell. This reaction consumes hydrogen ions. Thus a functional protein is expected to increase the overall pH of the cell.

Note: pH Readings were not taken for this composite part but for its sister part BBa_M36939 (lacks Comet GFP). We suspect that similar results would be obtained for this construct (containing Comet GFP). This data is included simply to provide a full analysis of the mechanism and its potential.

PHchart.jpg

Phgraph.jpg

Conclusion: Production of the target protein (enoyl-[acyl-carrier-protein] reductase) causes an increase in pH, as is to be expected. Therefore we conclude that the target protein is functional. This is statistically significant.

User Reviews

UNIQ54a8db9672749a86-partinfo-00000000-QINU UNIQ54a8db9672749a86-partinfo-00000001-QINU