Difference between revisions of "Part:BBa K1499250:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This GFP was designed with two TAG stop codons in place of leucine residues. | + | This GFP was designed with two TAG stop codons in place of leucine residues to only be expressed correctly in Amberless cells. |
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===References=== | ===References=== | ||
Lajoie, MJ <i>et al.</i> (2013) Genomically Recoded Organisms Impart New Biological Functions. <i>Science</i> 342: 357-60. 24136966 | Lajoie, MJ <i>et al.</i> (2013) Genomically Recoded Organisms Impart New Biological Functions. <i>Science</i> 342: 357-60. 24136966 | ||
Latest revision as of 20:42, 2 November 2014
GFP (E0040) with two amber stop codons
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 705
Design Notes
This GFP was designed with two TAG stop codons in place of leucine residues to only be expressed correctly in Amberless cells.
References
Lajoie, MJ et al. (2013) Genomically Recoded Organisms Impart New Biological Functions. Science 342: 357-60. 24136966