Difference between revisions of "Part:BBa K1499500:Experience"

Latest revision as of 19:33, 2 November 2014

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Applications of BBa_K1499500

Figure 1. Flurometer data for construct.

Figure 2. Flow cytometry data for construct.

To measure the time delay created by the quorum sensing construct we used GFP as a visual reporter and measured change in fluorescence with time after induction with IPTG. After using a fluorometer to obtain preliminary data on changes in GFP expression, we used flow cytometry to quantify this change. In the graphs above it is visible that, although GFP expression was leaky, fluorescence increased 10-fold after induction with IPTG. Thus, the quorum sensing construct can be used to create a time delay that increases expression of the gene following the luxPR promoter by an entire order of magnitude.

User Reviews

UNIQ8d12a5a94c671e36-partinfo-00000000-QINU UNIQ8d12a5a94c671e36-partinfo-00000001-QINU

@@ Line 5: / Line 5: @@
 ===Applications of BBa_K1499500===
+[[File:Flurorometer_Data.png|700px|thumb|center|<b>Figure 1.</b> Flurometer data for construct.]]
 [[File:SBS_QuorumSensingFacs.png|700px|thumb|center|<b>Figure 2.</b> Flow cytometry data for construct.]]
-To measure the time delay created by the quorum sensing construct we used GFP as a visual reporter and measured change in fluorescence with time after induction with IPTG. After using a fluorometer to obtain preliminary data on changes in GFP expression, we used flow cytometry to quantify this change. In the graphs above it is visible that, although GFP expression was leaky, fluorescence increased 10-fold after induction with IPTG. Thus, the quorum sensing construct can be used to create an 18 hour time delay that increases expression of the gene following the luxPR promoter by an entire order of magnitude.
+To measure the time delay created by the quorum sensing construct we used GFP as a visual reporter and measured change in fluorescence with time after induction with IPTG. After using a fluorometer to obtain preliminary data on changes in GFP expression, we used flow cytometry to quantify this change. In the graphs above it is visible that, although GFP expression was leaky, fluorescence increased 10-fold after induction with IPTG. Thus, the quorum sensing construct can be used to create a time delay that increases expression of the gene following the luxPR promoter by an entire order of magnitude.
 ===User Reviews===