Difference between revisions of "Part:BBa K1321096"

Latest revision as of 04:59, 2 November 2014

Linker-CBDcipA-Linker + Phytochelatin (PC) EC20

Synthetic phytochelatin EC20 (metal binding peptide) fused C-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.

At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA.

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below:

Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.

For reference the cellulose binding domain binding capability to bacterial cellulose was measured relative to other cellulose binding domains when fused to sfGFP, the data for which can be seen here - K1321371.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 148
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 148
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 148
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 148
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 148
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.
-For reference the cellulose binding domain binding capability to bacterial cellulose was measured relative to other cellulose binding domains when fused to sfGFP, the data for which can be seen here ([https://parts.igem.org/Part:BBa_K1321367 here])  - K1321371.
+For reference the cellulose binding domain binding capability to bacterial cellulose was measured relative to other cellulose binding domains when fused to sfGFP, the data for which can be seen [https://parts.igem.org/Part:BBa_K1321371 here]  - K1321371.
 <!-- Add more about the biology of this part here