Difference between revisions of "Part:BBa K1391107:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K1391107=== | ===Applications of BBa_K1391107=== | ||
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| + | This is fusion protein comprising Syk and tobacco etch virus (TEV) protease, joined by a glycine-serine linker. | ||
This part is under an inducible TRE promoter that requires the presence of both the activator rtTA and the antibiotic Doxycycline for transcription to occur. | This part is under an inducible TRE promoter that requires the presence of both the activator rtTA and the antibiotic Doxycycline for transcription to occur. | ||
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| + | Spleen Tyrosine Kinase (Syk) is a tyrosine kinase that has a high affinity for the phosphorylated immunoreceptor tyrosine-based activation motif (ITAM) of the CD79A and CD79B proteins (components of the B-Cell Receptor Complex). Syk binds to the phosphorylated ITAM and the ITAM then phosphorylates Syk allowing Syk to initiate a downstream signalling cascade that ordinarily results in the proliferation of B-Cells during clonal selection. | ||
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| + | TEV protease is a protease that cleaves at a particular amino acid sequence. In our synthetic B-cell receptor system, a transcriptional activator (Gal4VP16) is fused using a TEV protease cleavage site to the intracellular tails of CD79A and/or CD79B. When the Syk-TEVp fusion is recruited to our engineered receptor upon antigen binding, the TEV protease cleaves at its cleavage site on the CD79A and/or CD79B fusions and releases a transcriptional activator. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 23:19, 1 November 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1391107
This is fusion protein comprising Syk and tobacco etch virus (TEV) protease, joined by a glycine-serine linker. This part is under an inducible TRE promoter that requires the presence of both the activator rtTA and the antibiotic Doxycycline for transcription to occur.
Spleen Tyrosine Kinase (Syk) is a tyrosine kinase that has a high affinity for the phosphorylated immunoreceptor tyrosine-based activation motif (ITAM) of the CD79A and CD79B proteins (components of the B-Cell Receptor Complex). Syk binds to the phosphorylated ITAM and the ITAM then phosphorylates Syk allowing Syk to initiate a downstream signalling cascade that ordinarily results in the proliferation of B-Cells during clonal selection.
TEV protease is a protease that cleaves at a particular amino acid sequence. In our synthetic B-cell receptor system, a transcriptional activator (Gal4VP16) is fused using a TEV protease cleavage site to the intracellular tails of CD79A and/or CD79B. When the Syk-TEVp fusion is recruited to our engineered receptor upon antigen binding, the TEV protease cleaves at its cleavage site on the CD79A and/or CD79B fusions and releases a transcriptional activator.
User Reviews
UNIQ8c41ac87a9168489-partinfo-00000000-QINU UNIQ8c41ac87a9168489-partinfo-00000001-QINU