Difference between revisions of "Part:BBa K1539001:Experience"
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===Applications of BBa_K1539001=== | ===Applications of BBa_K1539001=== | ||
| − | BBa_K1539001 was created using devices BBa_K1539021 and BBa_K1539055 to insert a relatively high expressing promoter and a high efficiency RBS site in front of mCherry from BBa_J0650. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, YYY% of our cells expressed mCherry and the median intensity was 4X brighter than a low promoter/weak RBS combination (see part [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1539002]). Our raw data is summarized below: | + | BBa_K1539001 was created using devices BBa_K1539021 [[https://parts.igem.org/Part:BBa_K1539021]] and BBa_K1539055 [[https://parts.igem.org/Part:BBa_K1539055]] to insert a relatively high expressing promoter and a high efficiency RBS site in front of mCherry from BBa_J0650 [[https://parts.igem.org/Part:BBa_J0650]]. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, YYY% of our cells expressed mCherry and the median intensity was 4X brighter than a low promoter/weak RBS combination (see part [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1539002]). Our raw data is summarized below: |
"High Expression Combination" - Primers PH1/RH1: | "High Expression Combination" - Primers PH1/RH1: | ||
Latest revision as of 21:07, 1 November 2014
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Applications of BBa_K1539001
BBa_K1539001 was created using devices BBa_K1539021 [[1]] and BBa_K1539055 [[2]] to insert a relatively high expressing promoter and a high efficiency RBS site in front of mCherry from BBa_J0650 [[3]]. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, YYY% of our cells expressed mCherry and the median intensity was 4X brighter than a low promoter/weak RBS combination (see part [4]). Our raw data is summarized below:
"High Expression Combination" - Primers PH1/RH1:
File:140821 DMC mCherry PH2-RH2.pdf
Negative Control:
File:140821 DMC mCherry Promoterless.pdf
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