Difference between revisions of "Part:BBa K1539002:Experience"
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===Applications of BBa_K1539002=== | ===Applications of BBa_K1539002=== | ||
| + | BBa_K1539001 was created using devices BBa_K1539034 and BBa_K1539089 to insert relatively a low expressing promoter and low efficiency RBS site in front of mCherry from BBa_J0650. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, 91.6% of our cells expressed mCherry, and the median intensity was 4X dimmer than a high promoter/high RBS combination (see part [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1539001]). Our raw data is summarized below: | ||
| + | "Low Expression Combination" - Primers PL1/RL1: | ||
| − | [[File: | + | [[File:140821_DMC_mCherry_PL1-RL1.pdf]] |
| + | |||
| + | Negative Control: | ||
| + | |||
| + | [[File:140821_DMC_mCherry_Promoterless.pdf]] | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 21:00, 1 November 2014
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1539002
BBa_K1539001 was created using devices BBa_K1539034 and BBa_K1539089 to insert relatively a low expressing promoter and low efficiency RBS site in front of mCherry from BBa_J0650. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, 91.6% of our cells expressed mCherry, and the median intensity was 4X dimmer than a high promoter/high RBS combination (see part [1]). Our raw data is summarized below:
"Low Expression Combination" - Primers PL1/RL1:
File:140821 DMC mCherry PL1-RL1.pdf
Negative Control:
File:140821 DMC mCherry Promoterless.pdf
User Reviews
UNIQ6508a8c7f458ec80-partinfo-00000000-QINU UNIQ6508a8c7f458ec80-partinfo-00000001-QINU