Difference between revisions of "Part:BBa K1360003"
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This part is the coding sequence for a thermostable mannosidase, which specifically degrades mannan. | This part is the coding sequence for a thermostable mannosidase, which specifically degrades mannan. | ||
− | + | [[File:ManA1_decolorfour.png|thumb|]] | |
===Usage and Biology=== | ===Usage and Biology=== | ||
− | The | + | The ManA1 enzyme produced by this part can be used in pulp bleaching. It presents high activity under a wide range of temperature (55℃-99℃) and pH conditions (3-10.5). The optimum working condition is pH = 5.0, temperature = 75.4℃. |
===Design considerations=== | ===Design considerations=== | ||
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[[File:DNS_Synergy5.png|center|frame||As you can see, the real DNS color is darker, so we think they can work in synergy.]] | [[File:DNS_Synergy5.png|center|frame||As you can see, the real DNS color is darker, so we think they can work in synergy.]] | ||
+ | [[File:DNS_Absorbance.png|center|frame||We also collect the absorbance data of the DNS. In this DNS method, OD540 corresponds to reductive saccharide concentration, but due to equipment limitation, we used OD570 for plate reader data.]] | ||
+ | ===Extra=== | ||
+ | [[File:Decolorfour.png|center|frame|In 2014, team Tongji's project Decolor Four.]] | ||
+ | |||
+ | [[File:Principle_Bleaching.gif|center|frame||The reason why these three hemicellulase can reduce chlorine consumption is because they break down hemicellulose which connects lignin and cellulose. In this way, bleaching detergent can "get into" the pulp easier, and less chlorine can make the same effect. This benefits environmental protection.]] | ||
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Latest revision as of 04:36, 2 November 2014
endo-1,4-beta-mannosidase ManA1
This part is the coding sequence for a thermostable mannosidase, which specifically degrades mannan.
Usage and Biology
The ManA1 enzyme produced by this part can be used in pulp bleaching. It presents high activity under a wide range of temperature (55℃-99℃) and pH conditions (3-10.5). The optimum working condition is pH = 5.0, temperature = 75.4℃.
Design considerations
This part is only a coding sequence without expression regulators, and is compatible with RFC10 standards, and the codon is optimized for E.coli expression.
Lab Archives
Extra
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 552
Illegal BamHI site found at 1170 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 544
Illegal SapI.rc site found at 459