Difference between revisions of "Part:BBa K1413041"
 
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<partinfo>BBa_K1413041 short</partinfo>
 
<partinfo>BBa_K1413041 short</partinfo>
  
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This part is an origin of replication which needs pi protein to replicate
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This part is an origin of replication which needs pi protein to replicate.
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We used OriVR6Kgamma in BBa_K1413044 taken from pNK2.
  
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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<html>
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It can be used in combinaison with transposon system which does not required a replicative system like in the part  <a href="https://parts.igem.org/Part:BBa_K1413044">BBa_K1413044</a> .
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<span class='h3bb'><b>Sequence and Features</b></span>
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</html>
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===Sequence and Features===
 
<partinfo>BBa_K1413041 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1413041 SequenceAndFeatures</partinfo>
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This OriVR6Kgamma contained a mutation. The XbaI site present in the wild-type sequence was mutated to
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be RFC10 compatible. Using primers:
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<br/>Fw ORI Mut (transposas plasmid): GAGCCTTGAGACCTCTAGAGCAGACGTCATACTAGATAACTAGATC
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<br/>Rv ORI Mut: GACGCAGGAGACCACTAGTATAAGTGTTCCTGTGTCAC
  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K1413041 parameters</partinfo>
 
<partinfo>BBa_K1413041 parameters</partinfo>
 
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This part requieres production of pi protein from pir gene.  
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This part requires production of pi protein from pir gene.
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<span class='h3bb'>References</span>
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===References===
 
Rakowski SA, Filutowicz;Plasmid R6K Replication Control; M. Plasmid. 2013 May; 69(3)231-242
 
Rakowski SA, Filutowicz;Plasmid R6K Replication Control; M. Plasmid. 2013 May; 69(3)231-242

Latest revision as of 18:00, 2 November 2014

Mutation of OriVR6Kgamma origin of replication (ori)

This part is an origin of replication which needs pi protein to replicate. We used OriVR6Kgamma in BBa_K1413044 taken from pNK2.


Usage and Biology

It can be used in combinaison with transposon system which does not required a replicative system like in the part BBa_K1413044 .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

This OriVR6Kgamma contained a mutation. The XbaI site present in the wild-type sequence was mutated to be RFC10 compatible. Using primers:
Fw ORI Mut (transposas plasmid): GAGCCTTGAGACCTCTAGAGCAGACGTCATACTAGATAACTAGATC
Rv ORI Mut: GACGCAGGAGACCACTAGTATAAGTGTTCCTGTGTCAC

Functional Parameters

This part requires production of pi protein from pir gene.

References

Rakowski SA, Filutowicz;Plasmid R6K Replication Control; M. Plasmid. 2013 May; 69(3)231-242