Difference between revisions of "Part:BBa K1500010"

Latest revision as of 16:58, 1 November 2014

IPTG--GFP--EmrR

Contains an IPTG-sensitive promoter (BBa_J04500), with RBS.3 (BBa_00032), and reporter GFP (E0040) downstream with an additional RBS and the EmrR mutator gene. Using a fluctuation analysis mutation rate experiment, XL1Blue bacteria transformed with this construct yielded a mutation rate of 3.14E-09 per cell per culture although mutation rate is dependent on growth conditions and can vary depending on experimental setup. This plasmid appears to incur a growth penalty in transformants.

Raw data:

Observed mutation count determined by (http://www.keshavsingh.org/protocols/FALCOR.html) by putting in values. Observed mutation count corrected to actual mutation count by the correction factor (mact=(mobs*(1-z))/(z(ln(z)) with z being the fraction of cultures plated) as only a portion of each parallel culture was plated.

The above graph compares mutation rate of wild-type XL1Blue as well as XL1Blue bacteria containing this BioBrick.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1424
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 870

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 <partinfo>BBa_K1500010 short</partinfo>
-Contains an IPTG-sensitive promoter (BBa_J04500), with RBS.3 (BBa_00032), and reporter GFP (E0040) downstream with an additional RBS and the EmrR mutator gene. Using a fluctuation analysis mutation rate experiment, XL1Blue bacteria transformed with this construct yielded a mutation rate of 3.14E-09 mutations per cell per culture although mutation rate is dependent on growth conditions and can vary depending on experimental setup. This plasmid appears to incur a growth penalty in transformants.
+Contains an IPTG-sensitive promoter (BBa_J04500), with RBS.3 (BBa_00032), and reporter GFP (E0040) downstream with an additional RBS and the EmrR mutator gene. Using a fluctuation analysis mutation rate experiment, XL1Blue bacteria transformed with this construct yielded a mutation rate of 3.14E-09 per cell per culture although mutation rate is dependent on growth conditions and can vary depending on experimental setup. This plasmid appears to incur a growth penalty in transformants.
+Raw data:
+[[File:rawdata.jpg]]
+Observed mutation count determined by (http://www.keshavsingh.org/protocols/FALCOR.html) by putting in values. Observed mutation count corrected to actual mutation count by the correction factor (mact=(mobs*(1-z))/(z(ln(z)) with z being the fraction of cultures plated) as only a portion of each parallel culture was plated.
 [[File:important_graph.jpg]]
+The above graph compares mutation rate of wild-type XL1Blue as well as XL1Blue bacteria containing this BioBrick.
 <!-- Add more about the biology of this part here