Difference between revisions of "Part:BBa K1419005"
 
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<partinfo>BBa_K1419005 short</partinfo>
 
<partinfo>BBa_K1419005 short</partinfo>
  
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This part contains the Lamp2B coding sequence with a downstream clover (GFP) sequence for easy localization.
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This part contains the Lamp2B coding sequence with a rabies virus glycoprotein tag and in-fusion clover (GFP) sequence for easy localization of Lamp2B C-terminal modification.
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This part was used for determining the localization of C-terminal additions to Lamp2B. Clover takes the place of a DNA binding protein that will be used to target plasmids to the exosomal interior.
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<html><p><a href="https://static.igem.org/mediawiki/parts/6/68/Lamp2B2014iGEMDatasheet.JPG"><image src="https://static.igem.org/mediawiki/parts/6/68/Lamp2B2014iGEMDatasheet.JPG"; position="centre"; width="500"; height="600"; /> </a></p></html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
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[[File:Lamp2B2014iGEMDatasheet.JPG|200px]]  
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Datasheet for Part BBa_K1419005.
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<br>
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[[File:Lamp2B2014iGEMDatasheet.jpg|200px]]  
  
  

Latest revision as of 18:33, 1 November 2014

RVG-Lamp2B-Clover

This part contains the Lamp2B coding sequence with a rabies virus glycoprotein tag and in-fusion clover (GFP) sequence for easy localization of Lamp2B C-terminal modification.

This part was used for determining the localization of C-terminal additions to Lamp2B. Clover takes the place of a DNA binding protein that will be used to target plasmids to the exosomal interior.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 119
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 153