Difference between revisions of "Part:BBa K1351028"

Latest revision as of 06:32, 1 November 2014

LMU Bacillus RBS collection 1

This part was generated in RFC10, and has the following prefix and suffix:

             Prefix                                                                   Suffix
5' - GAATTC GCGGCCGC T TCTAGA G aaggagggata T ACTAGT A GCGGCCG CTGCAG - 3'
     EcoRI    NotI      XbaI                                         SpeI     NotI   PstI

This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].

This library was generated utilizing the Salis lab RBS calculator to find RBS that cover a high space of translation intitiation rate in 'B. subtilis'. It includes seven RBS with the following sequences:

Number	BBa_K1310 ..	Sequence
1	28	aaggagggata
2	29	agaggaggata
3	30	aaggagagata
4	31	aggagaggata
5	32	aagaggagata
6	33	agaaagggata
7	34	aagaagagata

The coverage of RBS strength as calculated by the RBS calculator is depicted in the Figure 1 shown below. It shows an evenly distributed strength spanning approxiamately three orders of magnitude.

Fig.1: Coverage of the translation initiation rate of the RBS library as calculated by the RBS calculator

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 19: / Line 19: @@
 !Sequence
 |-
-|1
+|<b>1</b>
-|28
+|<b>28</b>
-|aaggagggata
+|<b>aaggagggata</b>
 |-
 |2
@@ Line 50: / Line 50: @@
 The coverage of RBS strength as calculated by the [https://salis.psu.edu/software/ RBS calculator] is depicted in the Figure 1 shown below. It shows an evenly distributed strength spanning approxiamately three orders of magnitude.
-[[File:LMU_14_RBSlibrary.png|middle|Fig.1: Coverage of the translation initiation rate of the RBS library as calculated by the [https://salis.psu.edu/software/ RBS calculator]]
+[[File:LMU_14_RBSlibrary.png|thumb|center|400px|Fig.1: Coverage of the translation initiation rate of the RBS library as calculated by the [https://salis.psu.edu/software/ RBS calculator]]]