Difference between revisions of "Part:BBa K1523025"

(Created page with "__NOTOC__ <partinfo>BBa_K1523008 short</partinfo> It's a device which can reduce Cr(VI) The coding part of this device is K1523024, which is yieF gene from E.Coli K12. It can e...")
 
 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K1523008 short</partinfo>
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<partinfo>BBa_K1523025 short</partinfo>
  
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It's a device which can reduce Cr(VI)
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It's a device which can reduce Cr(VI) from P. putida.
  
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The coding part of this device is K1523024, which is yieF gene from E.Coli K12. It can express the flavoprotein as a kind of chromate reductase.
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It is called yieF gene in E.Coli K12. It can express the flavoprotein as a kind of chromate reductase.
  
 
Involved in the protection against chromate toxicity. Catalyzes the transfer of three electrons to Cr6+ producing Cr3+ and one electron to molecular oxygen without producing the toxic Cr5+ species and only producing a minimal amount of reactive oxygen species (ROS). It can also reduce quinones, potassium ferricyanide, 2,6-dichloroindophenol, V5+, Mo6+, methylene blue and cytochrome c. The quinone reductase activity may protect against oxidative stress by preventing redox cycling of quinones which would otherwise generate ROS and by maintaining a pool of reduced quinone in the cell that is able to quench ROS directly. It is able to use both NAD or NADP equally well.
 
Involved in the protection against chromate toxicity. Catalyzes the transfer of three electrons to Cr6+ producing Cr3+ and one electron to molecular oxygen without producing the toxic Cr5+ species and only producing a minimal amount of reactive oxygen species (ROS). It can also reduce quinones, potassium ferricyanide, 2,6-dichloroindophenol, V5+, Mo6+, methylene blue and cytochrome c. The quinone reductase activity may protect against oxidative stress by preventing redox cycling of quinones which would otherwise generate ROS and by maintaining a pool of reduced quinone in the cell that is able to quench ROS directly. It is able to use both NAD or NADP equally well.
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'''Reference'''
 
'''Reference'''
 
Uniprot : http://www.uniprot.org/uniprot/H0QEW2
 
Uniprot : http://www.uniprot.org/uniprot/H0QEW2
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K1523008 SequenceAndFeatures</partinfo>
 
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===Functional Parameters===
 
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<partinfo>BBa_K1523008 parameters</partinfo>
 
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Latest revision as of 16:21, 31 October 2014

ChrR coding sequence

It's a device which can reduce Cr(VI) from P. putida.

It is called yieF gene in E.Coli K12. It can express the flavoprotein as a kind of chromate reductase.

Involved in the protection against chromate toxicity. Catalyzes the transfer of three electrons to Cr6+ producing Cr3+ and one electron to molecular oxygen without producing the toxic Cr5+ species and only producing a minimal amount of reactive oxygen species (ROS). It can also reduce quinones, potassium ferricyanide, 2,6-dichloroindophenol, V5+, Mo6+, methylene blue and cytochrome c. The quinone reductase activity may protect against oxidative stress by preventing redox cycling of quinones which would otherwise generate ROS and by maintaining a pool of reduced quinone in the cell that is able to quench ROS directly. It is able to use both NAD or NADP equally well.

Catalytic activity

NAD(P)H + a quinone = NAD(P)+ + a hydroquinone.

2 NAD(P)H + Cr6+ + O2 = 2 NAD(P)+ + Cr3+ + H2O2.

Structure

Structure yieF.jpg

This picture is from RCSB

Reference Uniprot : http://www.uniprot.org/uniprot/H0QEW2