Difference between revisions of "Part:BBa K1413044:Experience"

Latest revision as of 18:08, 2 November 2014

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 how you used this part and how it worked out.
-===Applications of BBa_K1413044===
-To replicate this new plasmid we can used E. coli.
-To separate the pSB1C3 and new pNK2 we must used BglII. After that the disgest product must migrate in the electrophoresis gel.The band to 2,6 kb , which is the biggest correspond to transposon plasmid.
-The transposon plasmid can be extract in the gel. The T4 ligase is used to circularize the plasmid.
-All the biobrick can introduce between the Is10 with biobrick enzyme. Also this biobrick can be integrate in Pseudovibrio denitrificans the genome with electro transformation.
-The voltage used is 2000V and the incubation time after the electroporation is to 3h.
-<html>
-<img src="https://static.igem.org/mediawiki/parts/c/ca/Kanamycine_gel_%281%29.png" width=50%/>
-</html>
-Picture of an electrophorese gel samples come from the PCR which amplify the kanamycineR cassette. All clones have the insertion of the transposon. The Control, Pseudovibrio denitrificans, do not the insertion as expected.
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-<img src="https://static.igem.org/mediawiki/2014/d/df/Tranposons.png" width=50%/>
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