Difference between revisions of "Part:BBa K1523022"
 
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__NOTOC__
 
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<partinfo>BBa_K1523022 short</partinfo>
 
<partinfo>BBa_K1523022 short</partinfo>
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nahE
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The gene encoding trans-o-hydroxybenzylidenepyruvate hydratase-aldolase (tHBP-HA) was isolated from Pseudomonas fluorescens N3, an environmental strain able to degrade naphthalene.
 
The gene encoding trans-o-hydroxybenzylidenepyruvate hydratase-aldolase (tHBP-HA) was isolated from Pseudomonas fluorescens N3, an environmental strain able to degrade naphthalene.
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[[File:Pathway_Naphthalene.jpg]]
 
[[File:Pathway_Naphthalene.jpg]]
  
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The F step is ctalyzed by this part K1523022
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The F step is ctalyzed by this part K1523022'''
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Reference:
 
Reference:
 
UniProt: http://www.uniprot.org/uniprot/Q51947
 
UniProt: http://www.uniprot.org/uniprot/Q51947
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"Organization and evolution of naphthalene catabolic pathways: sequence of the DNA encoding 2-hydroxychromene-2-carboxylate isomerase and trans-o-hydroxybenzylidenepyruvate hydratase-aldolase from the NAH7 plasmid."
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Eaton R.W.
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J. Bacteriol. 176:7757-7762(1994)
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:21, 31 October 2014

nahE coding sequence


The gene encoding trans-o-hydroxybenzylidenepyruvate hydratase-aldolase (tHBP-HA) was isolated from Pseudomonas fluorescens N3, an environmental strain able to degrade naphthalene.

Naphthalene is a kind of pollution source. nahE is a member of the naphthalene catabolic in Pseudomonas putida

Function

Involved in the naphthalene upper catabolic pathway. Catalyzes the transformation of trans-O-hydroxybenzylidenepyruvate (THBPA) to salicylaldehyde and pyruvate. The reaction is reversible. Can also use substrate which carry trans-alpha,beta-unsaturated keto acid side chain and adjacent hydroxyl group such as trans-4-(3-hydroxy-2-thianaphthenyl)-2-oxo-but-3-enoate, trans-4-(3-hydroxy-2-benzofuranyl)-2-oxobut-3-enoate, and trans-4-(3-hydroxy-2-thienyl)-2-oxobut-3-enoate

Pathway Naphthalene.jpg


The F step is ctalyzed by this part K1523022



Reference: UniProt: http://www.uniprot.org/uniprot/Q51947

"Organization and evolution of naphthalene catabolic pathways: sequence of the DNA encoding 2-hydroxychromene-2-carboxylate isomerase and trans-o-hydroxybenzylidenepyruvate hydratase-aldolase from the NAH7 plasmid." Eaton R.W. J. Bacteriol. 176:7757-7762(1994)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 631
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 742
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 256
    Illegal SapI.rc site found at 148