Difference between revisions of "Part:BBa K1471007:Design"
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− | + | ===Design Notes=== | |
− | + | We have optimized the ''mer'' proteins for ''Arabidopsis thaliana'' to achieve the highest translation levels. The Ribosome Binding Sites we used are from the genome of the same organism: ''A. thaliana''. | |
− | + | ===Source=== | |
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− | '' | + | ''Arabidopsis thaliana'' genome. |
− | + | ===References=== | |
+ | Das S., Dash H. R., (2012). Bioremediation of mercury and the importance of bacterial mer genes. National Institute of Technology.India: International Biodeterioration & Biodegradation. Volume 75. Pages 207-213 | ||
− | + | Kiyono M. , et al (2013) Increase methylmercury accumulation in Arabidopsis thaliana expressing bacterial broad-spectrum mercury transporter MerE. Springer. Issue 3; Pages 1-13 | |
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− | + | Kiyono M., Sone Y., Nakamura R., et al (2013) Role of MerC, MerE, MerF, MerT, and/or MerP in Resistance to Mercurials and the Transport of Mercurials in Escherichia coli. Biological and Pharmaceutical Bulletin. Volume 36; Issue 11; pages 1835-1841 | |
− | + | Comba P. (2011). Modeling of Molecular Properties. Weinheim,Germany:Wiley-VCH. Pages 313-320 Bizily S., Rugh C., Summers A., Meagher R. (1999). Modeling of Molecular Properties. University of Georgia, USA. | |
− | + | Rangan, et al. (2008). Analysis of Context Sequence Surrounding Translation Initiation Site from Complete Genome of Model Plants. New York University. [Online] Retrieved october 14th 2014 from: http://www.nyu.edu/projects/vogel/Reprints/Rangan_MolBt08.pdf | |
− | + | Nakagawa, et al. (2007). Diversity of preferred nucleotide sequences around the translation initiation codon in eukaryote genomes. Oxford University Press. [Online] Retrieved october 14th 2014 from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2241899/ | |
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− | + | Liu Q, Xue Q. (2005). Comparative studies on sequence characteristics around translation initiation codon in four eukaryotes. Zhejiang University. [Online] Retrieved october 14th 2014 from: http://www.ias.ac.in/jgenet/Vol84No3/317.pdf | |
− | + | Kozak, M. (1989). Circumstances and mechanisms of inhibition of translation by secondary structure in eucaryotic mRNAs. American Society for Microbiology (ASM). [Online] retrieved october 14th 2014 from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC363665/ | |
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Latest revision as of 21:28, 2 November 2014
RBS - MerB - RBS - MerE.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 428
Illegal AgeI site found at 375 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We have optimized the mer proteins for Arabidopsis thaliana to achieve the highest translation levels. The Ribosome Binding Sites we used are from the genome of the same organism: A. thaliana.
Source
Arabidopsis thaliana genome.
References
Das S., Dash H. R., (2012). Bioremediation of mercury and the importance of bacterial mer genes. National Institute of Technology.India: International Biodeterioration & Biodegradation. Volume 75. Pages 207-213
Kiyono M. , et al (2013) Increase methylmercury accumulation in Arabidopsis thaliana expressing bacterial broad-spectrum mercury transporter MerE. Springer. Issue 3; Pages 1-13
Kiyono M., Sone Y., Nakamura R., et al (2013) Role of MerC, MerE, MerF, MerT, and/or MerP in Resistance to Mercurials and the Transport of Mercurials in Escherichia coli. Biological and Pharmaceutical Bulletin. Volume 36; Issue 11; pages 1835-1841
Comba P. (2011). Modeling of Molecular Properties. Weinheim,Germany:Wiley-VCH. Pages 313-320 Bizily S., Rugh C., Summers A., Meagher R. (1999). Modeling of Molecular Properties. University of Georgia, USA.
Rangan, et al. (2008). Analysis of Context Sequence Surrounding Translation Initiation Site from Complete Genome of Model Plants. New York University. [Online] Retrieved october 14th 2014 from: http://www.nyu.edu/projects/vogel/Reprints/Rangan_MolBt08.pdf
Nakagawa, et al. (2007). Diversity of preferred nucleotide sequences around the translation initiation codon in eukaryote genomes. Oxford University Press. [Online] Retrieved october 14th 2014 from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2241899/
Liu Q, Xue Q. (2005). Comparative studies on sequence characteristics around translation initiation codon in four eukaryotes. Zhejiang University. [Online] Retrieved october 14th 2014 from: http://www.ias.ac.in/jgenet/Vol84No3/317.pdf
Kozak, M. (1989). Circumstances and mechanisms of inhibition of translation by secondary structure in eucaryotic mRNAs. American Society for Microbiology (ASM). [Online] retrieved october 14th 2014 from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC363665/